234份芥菜型油菜种质苗期耐低磷性综合评价

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Abstract： To evaluate the phosphorus deficiency tolerance of Brassca juncea （L.） Czern.et Coss at the seeding stage，we investigated12 traits，such as shoot dry weight，root dry weight，shoot phosphorus concentration，root phosphorus concentration，across 234 germplasms under normal phosphorus （ KH2PO4. ）and low phosphorus（ 5μmol/LKH2PO4 ） treatments.The phosphorus deficiency tolerance coefficient wascalculated for each trait，and principal component analysis，fuzzy membership function analysis，and cluster analysis wereused for a comprehensive evaluation.The results revealed substantial variations in all seedling traits under both treatments. If compared to those under normal phosphorus treatment，low phosphorus treatment led to reductions in dry weight，phosphorus concentration and phosphorus accumulation in both shoots and roots.In contrast，the root-shoot ratioand phosphorus use eficiency in shoots and roots increased.Through principal component analysis，we transformed the phosphorus deficiency coeffcientsof the12 traits into four principal components，which collectively accounted for 96.511% of the total variance.Based on component weights，a comprehensive D -value was calculated. Cluster analysis classified the 234 germplasms into four groups：phosphorus-deficient tolerant（10 germplasms），slightlyphosphorus-deficient tolerant （53 germplasms），slightly phosphorus-deficient sensitive（105 germplasms），and phosphorus-deficient sensitive （67germplasms）．The phosphorus-deficient tolerant accessions exhibited significantly higher tolerance coeficients for root dry weight，root-shoot ratio，shoot phosphorusaccumulation，root phosphorus accumulation and plant phosphorus accumulation if compared to the other groups.This study provides a reference forevaluating phosphorus deficiency tolerance in B. juncea and identifiesvaluable genetic resources for uncovering related genes and developing phosphorus-deficient varieties.

Key words： Brassica juncea ;low phosphorus stress;germplasm evaluation

磷（P）是作物生长发育所必需的元素之一，它不仅是核酸、蛋白质和磷脂等生物大分子不可或缺的结构成分，还是介导磷酸盐信号网络的关键信号因子，参与植物体内诸多代谢活动和细胞调节过程[3]。（剩余16458字）