沼液施用下的西蓝花种植土壤微生物群落演变特征

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中图分类号：S154.3；S635.3 文献标志码：A 文章编号：1672-2043（2025）12-3172-09 doi：10.11654/jaes.2024-1061

Evolution characteristics of soil microbialcommunities inbroccoli cultivationunder biogas slurry application

ig'，MENGQingting，FUJiajie，JINYuqing'，CAIJianjun1，MAJiawei，WANGMei （1.LinhaiAgriculturalTechnologyPromotion Center，Linhai317ooo，China;2.CollgeofAnimalScienceandTechnology ⋅ College of VeterinaryMedicine，ZhejiangA&FUniversityHangzou3113oo，China;3.CollgeofTeaScienceandTeaCulture，ZhejangA&F University，Hangzhou311300，China）

Abstract：Toinvestigateteipactofbiogassurraplicationontediversityofacterialandfungalcommunitiesinsilsusedfor broccolicultivation.Usingmetagenomicsequencingtechnologyweanalyzedthechangesinbacterialandfungaldiversitydertwo treatments：combiedplicationofhemicalfertlizerandogassurrMX），andsoleapplicationofiogassuBS）.Cobindith soilphsicochemicalpropertis，efurthervealedteevolutioarypatersofcrobialcommuits.Teresultsosthat：Coad withtheconrK），eratantleasdl，slaceO）lbleoAK），dlbl phosphorus（AP），with AK and AP content increasing by 633.0% and 95.3% ，respectively.In contrast，the MIX treatment reduced soil pH butenhancedtotalnitrogen（T）andAPlevels.Microbialcommunityanalysisrevealedthat，inermsofbacteria，theBStreatment markedlyincreasedtherelativeabundancesofPseudomonadotaandChloroflexota，withChloroflexotashowinganotableincreaseof 229.6% compared toCK.TheMIXtreatment，ontheotherhand，significantlyenrichedBacteroidota.Forfungi，theBStreatment significantly promoted the proliferation of Ascomycota，witha 148.7% increase in abundance，while the MIX treatment enriched

2025年12月

OlpidiomycotapopulatiosinthesoilCoelationaalysisidicatedtatteabundanceofShingomonasaspositivelyoelaedwith SOMandAK，ileteaundanceofAspergllireasdwithigerpHutasnegativelyelatedwithalalinedrolisogn （AHN）adtotalpospous（T）.Ioclusi，tplicatioogassuyificatlyprovdsilp，O，AK，ndels， whilesimultaneouslyoptimiingthesoilmicrobialcommunitystructure.Thesechanges enhancednutrientcyclinganddiseasesueion potentialinbacterialandfungalomuitisAsviroentallfrndlyganicfertilieiogassuroldsgeatoisefor improving soil quality and promoting sustainable agricultural development.

Keywords：biogas slurry; broccoli; soil bacterial community; soil fungal community

西蓝花是一种营养价值极高的蔬菜，富含维生素C、维生素E、槲皮素和萝卜硫素等多种活性成分，这些成分具有显著的抗氧化、抗炎及降低慢性疾病风险的潜力[1-2]。（剩余19578字）