不同黏土矿物对恶臭假单胞菌KT2440生物膜形成的影响机制

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关键词：黏土矿物；恶臭假单胞菌；生物膜；氧化应激反应；代谢活性中图分类号：S15 文献标志码：A 文章编号：1672-2043（2025）12-3162-10 doi：10.11654/jaes.2025-0220

Mechanism of different clay minerals influencing the biofilm formation of Pseudomonas putida KT2440

JIANG Yu，LIU Shanpeng，TANG Yuqing， ZHAO Sheng，XIONG Xuan， YAN Guili， OUYANG Kai* （College ofResources，Hunan Agricultural University，Changsha 41O128，China）

Abstract：Toelucidatetheinfluencemechanismsofclaymineralsinsoilonthegrowthanddevelopmentofbacterialioflms.Thisstudy employedscientificinstrumentsincludingamultifunctionalmicroplatereaderandfluorescencemicroscope，combinedwithtechnical approachessuchascrystalvioletstainingandfluorescencedetection，toinvestigatetheefectsoftreetypicalsoilclayineals （montmorilonite，kaolinite，ndgoethite）onthegrowthofPseudomonasputidaKT2440andbiflmformation.Theresultsdeostrated that，compared tothepureculturesystem，thebiofilm biomassinthemontmorillonite-treatedsystem increasedby78.95 % .Withinlow concentration thresholds，goethite （ 0-5mg⋅L-1 ）andkaolinite （ 0-100mg⋅L-1 ）both promoted bacterial biofilm formation to varying degrees，while high concentrations of goethite（ >5mg⋅L-1 ）andkaolinite（ >100mg⋅L-1 ）significantlyinhibitedbiofilmformation，with maximum inhibition rates of 83.78% and 34.72% ，respectively. Through co-sedimentation experiments and fluorescence microscopy observatins，itwasdemonstratedtatgoethitecaneectivelyadsorbandkillbacteria.Teproductionofreactieoxygespeies（O） inducedbygoetiteandaoiites3.66tisand1.97ieshighespectivelytantatinthepureculuresstem.Addaly after 192 h of co-cultivation with P .putida，the intracellular adenosine triphosphate（ATP）content in the goethite-treated system decreased by 58.21% compared to the pure culture system.Montmorillnite mitigates bacterialcell membranedamage through electrostatic repulsionandancesbacterialcozatioviitsghspcificsufacareaterebysignificantlypromotingbacterialgrowthadbiofil formatioIosiedoiteatlooeatiosillubeoflleasiutrttatuteoo whereasathighoncentratios，tyinduceoxidativesres，disuptcellularmetabolicfunctios，nduppressiofideelopt. Keywords：clay minerals; Pseudomonas putida; biofilm; oxidative stress response; metabolic activity

土壤活性组分是指土壤中化学和生物活性较高、易参与地球化学过程，对植物和微生物有较强有效性的部分物质，主要包括腐植酸、黏土矿物和铁/锰/铝氧化物等。（剩余20667字）