黄芩转录组SNP和InDel位点的挖掘及特征分析

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中图分类号：R2 文献标志码：A 文章编号：1674-490X（2025）06-0034-07

Identification and characterization of SNP and InDel variants in Scutellaria baicalensis Georgi transcriptom

SUN Zhichao'，ZHAO Chunying²，LU Yang²，XIE Yan¹，WANG Hui1，GAO Yanxia1 （1.Institute of Sericulture，Chengde Medical University，Chengde O67O0O，China；2.Institute of Chinese Materia Medica，Chengde Medical University-Hebei Key Laboratory of Research and Development for Traditional Chinese Medicine，Chengde O67OoO，China）

Abstract： Objective To develop specific molecular markers of Scutellaria baicalensis and perform genetic diversity analysis and molecular marker-asssted breeding. Methods Single nucleotide polymorphisms（SNP） and insertion and deletion polymorphisms （InDel） sites in the transcriptome of flowers and roots of Scutellaria baicalensis were analyzed. Results The results showed that an average of 216 067 SNPs were found in the transcriptome of 18 skulcap flowers and roots，and the ratio of conversion to transversion was 1.42～1.61，and the number of loci distributed in the exome region was the largest， and the number of loci with missense mutations was the largest. The average number of InDel loci in each sample was 22 864，and the number of insertion loci was greater than that of the deletion type， which was the most distributed in the gene and had the most frameshift insertions. Conclusion The results show that the transcriptome of the flowers and roots of Scutellaria baicalensis is rich in SNPs and InDel loci，and has high polymorphisms，which provides data support for the development of specific molecular markers in Scutellaria baicalensis.

Key Words： Scutellaria baicalensis Georgi； transcriptome; SNP； InDel

随着高通量测序技术的不断发展和成本逐渐降低，转录组测序技术在植物育种中得到广泛应用。（剩余5260字）