西双版纳茶区茶轮斑病的致病病原分离及鉴定

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中图分类号：S571.1；S435.711 文献标识码：A 文章编号：1000-369X（2025）06-1047-08

Abstract：Tea grayblight isoneof the major foliardiseases intea plantations，significantlyaffecting both yieldand quality. In July 2023，tea leaves exhibiting resembling gray blight symptoms were observed during a filed surver in tea plantations in Mengla County，Xishuangbanna Dai Autonomous Prefecture，Yunnan Province.As the causal pathogen of these symptoms had not been previously reported in this region，this study aimed to identify the causal agent and its taxonomic classification.The pathogen was successfully isolatedand identified using a combination of tissue isolation，pathogenicity testing via wound inoculation，morphological characterization，and molecular phylogenetic analysis based on ITS and TUB genes squences.The results confirmed that the strainFN3-2，isolated from symptomatic leaves， was pathogenic. Based on morphological traits and molecular evidence，the pathogen was finally identified as Neopestalotiopsis protearum.The finding provided a scientific basis for theaccurate disease diagnosis and biocontrol agent screening，contributing to the sustainable development of tea production in the region. Keywords： tea， gray blight， Neopestalotiopsis protearum， pathogen identification

茶树[Camellia sinensis（L.）O.Kuntze]属山茶科（Theaceae）山茶属（Camellia），为多年生常绿木本植物，是一种重要的经济作物，喜酸性土壤和暖湿气候[1-2]。（剩余12732字）