茶树CsPDAT1基因克隆及抗旱性分析

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中图分类号：S571.1；S435.711 文献标识码：A 文章编号：1000-369X（2025）06-0920-11

Cloning of the CsPDAT1 Gene from Camellia sinensis and Its Role in Drought Tolerance

SHEN Yingzi， LI Duojiao， HU Xingrong， JIANG Li， ZHENG Zhaisheng， LIU Huikang， YUAN Ming'an JinhuaAcademyofAgriculturalSciences，Jinhua32lol7，China

Abstract： During the growth and development of tea plants （Camelia sinensis），abiotic stresses such as drought and salinity frequently occur，causing significant adverseeffctson tea yield and quality.This study focused onthe tea phospholipid： diacylglycerol acyltransferase 1 （PDAT1） gene andsystematically investigated its function in response to abiotic stresses through bioinformatics analysis，gene expression pattern detection，and transgenic functional verification. Bioinformatics analysis reveals that the protein encoded by the CsPDAT1 gene posssses a typical lysophospholipid acyltransferase （lysoPLA）domain.Real-time quantitative PCR （qRT-PCR）results demonstrats that the expresion of the CsPDATl gene was significantly upregulated under drought and high-salt treatments， particularly during theearly stages of drought stressTo further elucidate its function，researchersconstructedan overexpression vector for the CsPDATl gene and transformed it into Arabidopsis thaliana.Finally three homozygous T3-generation transgenic lines were obtained.Phenotypic analysis reveals that under drought stres，Arabidopsis plants overexpressing the CsPDAT1 gene exhibited a significantly higher seed germination rate compared to that of wild-type plants，along with reduced malondialdehyde （MDA） content，increased proline content，and significantly enhanced antioxidant enzyme activity.This study confirmed that the CsPDATl gene in tea plants enhances plant tolerance to abiotic stressbyregulating cellmembrane lipid metabolismand the accumulation of osmoprotectants， providing a theoretical foundation and genetic resources for stress-resistant molecular breeding in tea plants. Keywords： tea plant， CsPDATl，gene cloning， drought resistance

茶树[Camellia sinensis （L.） O.Kuntze]作为全球重要的经济作物，为众多国家和地区带来了可观的经济收益。（剩余12654字）