柚和茶树上柑橘始叶螨线粒体基因组特征及叶螨科系统发育分析

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中图分类号：S571.1；S435.711

文献标识码：A

文章编号：1000-369X（2025）06-1021-15

Mitochondrial Genome Characteristics and Phylogenetic Analysis of Eotetranychus kankitus on Pomelo and Tea Plants

CHEN Shichun， JIANG Hongyan， LIAO Shuran， CHEN Tingxu， WANG Xiaoqing Tea Research Institute of Chongqing Academy ofAgricultural Sciences， Chongqing 402160，China

Abstract：Eotetranychus kankitus isanagricultural pest mitethat damages manycrops.Conducting mitochondrial genome analysis can clarify its structural characteristics and the phylogenetic relationships among species in the family of Tetranychidae species. The mitochondrial genome sequences of E .kankitus on pomelo and tea plantswere obtained by PCR amplification，sequencing，assemblyand annotation.The phylogenetic tree of Tetranychidae was constructed based on13 protein-coding genes and 2 rRNA gene sequences by maximum likelihood and Bayesian methods.The mitochondrial genomes of E .kankituson pomelo and tea plants were both in size of 13 O32 bp，with GenBank accesion numbers OQ955767and OQ955768，respectively.Both the two mitochondrial genomes included 13 protein coding genes，2 rRNA genes，22 tRNA genes and a 40 bp control region， with aconsistent gene arrangement.The sequence similarity rate of the two mitochondrial genomes was high，and base composition values of the whole genome and each region were close. E . kankitus on both hosts clustered into one branch in the phylogenetic tree，and the genetic distance between them was 0.0680 ，which was much smaller than that between E .kankitus and other mites in Tetranychidae.A close relationship was showed between E .kankitus and Panonychus species in both genetic distance and phylogenetic analysis.According to analyses of mitochondrial genomes，the diferentiation degree of E kankitus on pomelo and tea plants was low，and the risk of transference between the two hosts was high. It is recommended that tea gardens with E .kankitus and surrounding citrus crops should strengthen field monitoring work. Keywords： Eotetranychus kankitus， Tetranychoidae， mitochondrial genome， phylogeny

柑橘始叶螨（EotetranychuskankitusEhara），又名四斑黄蜘蛛、柑橘黄蜘蛛，隶属于蛛形纲（Arachnida）蜱螨亚纲（Acari）蜱螨目（Acarina）叶螨科（Tetranychoidae），主要危害柑橘类作物，是我国一些柑橘产区的重要害螨，给种植户造成了严重损失[1]。（剩余21559字）