穿心莲内酯调节AMPK/NLRP3信号通路对高糖诱导的心肌细胞焦亡的影响

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EffectndrographolideonHighGlucoseInducedPyroptosisMyocardialCelsbyRegulatingtheAMPK/NP3SignalingPathway SUNXue，LIUHuimin，RUANHualing，ZHANGBingfei

Corresponding AuthorZHANG Bingfei，E-mail： z86uua @ 163.com

AbstractObjective：Toinvestigatetheefectandrographolide（AD）onhighglucose（HG）inducedpyroptosismyocardialcelsby regulatingtheadenosinemonopospateactivatedproteinkinaseAKodikeeceptorprotein3（P3）ignalingpathayetos： Logarithmic phase H9c2 cells were divided into control group，HG group（50 mmol/L HG）， HG+AD-L group 30mg/L AD+50mmol/L HG），HG+AD-M group 40mg/L AD+50 mmol/L HG），HG+AD-H group （50mg/L AD+50 mmol/L HG），and HG+ADH+AMPK inhibitor Compound C（CC ）970μρ（50mg/↕ΔD+50mmol/↕HG+10μmol/L CC）.CCK-8 method and Hoechst 33342/propidium iodide（Pl） stainingwereusedtodeterinetheproiferationactityandcellpyoptosisH9c2cell.Enzelinkedimmunosorbentassy） methodwasapliedtodeterminethelevels lactatedehydrogenase（LDH）andinflammatoryfactors.Reversetranscriptionpolymerase chainreactionqR-PCR）wasapiedtomeasurethemRNAexpressioncellpyroptosisfactor.WestemBlotwasapliedtodetermine the expression AMPK/NLRP3 pathway proteins and pyroptosis proteins.Results： 10-60mg/L AD was able to enhance the HG-induced proliferation activity H9c2 cells，andafter interventionwith 50mg/L AD，the proliferation activity H9c2 cels was the highest. Therefore，30，40，and 50mg/L AD were selected for subsequent experiments.Compared with the control group，the OD570 value and p-AMPK/AMPKintheHGgroupdecreased，whilethecellpyroptosisrate，DH，levelsinflammatoryfactors，mRNAandprotein expression pyroptosis factors increased（ P<0.05 .Comparedwiththe HG group，the OD570 value and p-AMPK/AMPK in the HG+AD-L group，HG+AD-Mgroup，andHGAD-Hgroupalincreased，whiletheremaining indicatorsmentionedabovedecreased P<0.05） Compared with the HG+AD+H group，the OD570 value and p -AMPK/AMPK in the HG+AD-H+CC group decreased，while the remaining indicatorsaboveallincreased P<0.05 ）.Conclusion：AD canalleviate HG-induced inflammatory damage to H9c2 cels，inhibit cell pyroptosis，and this effect may be related to regulating the AMPK/NLRP3 signaling pathway.

Keywordscellpyroptosis；myocardialcell;highlucoseandrographolide;AMPactivatedproteinkinase/Nod-ikereceptorteal proteindomainassociatedprotein3;experimentresearch

糖尿病（DM）是以血糖代谢紊乱为特征的慢性疾病，长期高糖（HG）环境可影响心肌微循环和心肌纤维化等，增加心血管疾病发生风险[1]。（剩余8948字）