香菇9608脱除LeV-HKB病毒后对子实体性状的影响分析

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中图分类号：S646.1 文献标志码：A DOI编码：10.3969/j.issn.1006—6500.2025.07.004

fectofLeV-HKBRemovalonFruitingBodyTraitsofShiitakeMushroom9608

LI Mengchun1， YUAN Chaozheng'， MA Yu1 ， YANG Yuhua'， ZHANG Zhifeng2， BAI Mingfeng³ （1.NanyangAcademyofSiences，Nanyang，Henan473O3，Chia;2.yngNoalUniversityaang，Henan473in; 3. Xixia EdibleFungi Research Center，Xixia County，Xixia，Henan 47455o， China）

Abstract：Toinvestigatetheefectof mushroomvirusLev-HKBonthedevelopmentofmushroomfruitingbodies.Inthisstudythe mainvarietyofshitake mushrooms inNanyang，96O8wasusedasthetestmaterial.Ribavirindrugdetoxificationcombined with mycelialtipdetoxificationtechnologywasusedtoobtainshitakemushroomstrainsthatwerefreeofLeVHKBvirus.Bysystematialy comparingandanalyingthemorpholgyyield，andothertraitsofthefruitingbodiesofdetoxifiedstrainsandvirus-fectedcotol strains，andusingmulti-layerperceptronandnearestneighbormethodtoanalyzethemainepigenetictraitsthatafecttraitidicators. Theresultsshowedthatthedetoxifedshitake mushrooms exhibitedsignificantincreasesinallproductiontraits.Theaverageyieldofa single mushroom increased by56.65 % ，thediameterofthefruitingbodycapsignificantlyincreasedby19.89 % ，thelength of the stem increased by 6.94% ，and the morphology became more complete.The length of the fungal stem was significantly correlated with detoxification，wilicatsereglyoelaedittoationouhiteactiveoelaonaalys，iasfoudat detoxificationhadasignificantpositivecorelationwiththeweightofindividualshitakemushroomsandasignfcantpositivecorelationwithyieldinrease.Multilayerperceptronandarestneigbornalysishoedatcapameter，capickness，andelength werthemainepigenetictraitsthatafectedothertraitindicators.Inconclusion，researchhasconfirmedthatthepresenceofLeV HKBcaninhibitthenoraldevelopmentofthefruitingbodyof96O8shitakemushrooms.Removingtheviruscanefectivelyimprove thefruitingbodytraits，providingtheoreticalbasisandtechnicalreferenceforhigh-qualityandhigh-yieldcultivationofhtake mushrooms and virus prevention and control.

Keywords：shiitakemushrooms;LeV-HKB;subentity;trait

香菇9608品种由河南省西峡县食用菌科研中心于1996年选育而成，在河南省南阳地区主栽近30年，为低温长菌龄品种，于2008年获得国家认定。（剩余7023字）