稻瘟菌侵染对水稻氮素吸收利用途径相关基因表达的影响

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中图分类号：S511：Q781 文献标识号：A 文章编号：1001-4942（2025）09-0031-1

Effects Magnaporthe oryzae Infection on Expression Genes Related to Nitrogen Absorption Utilization Pathways Rice

Huang Hao，L Jjun，Chen Wenfeng，Yao Wei，Chen Qianru， Huang Xilai， Wu Jun，Liu Xionglun，Liu Jlg （Colege Cha/Hunan Provcial Laboratory Crop Gene Engeerg， Changsha ，Cha）

AbstractRice blast is the most devastatg fungal disease rice production. High nitrogen fertilizer application tends to promote rice susceptibility to blast，however，the underlyg genetic basis remas unclear. To elucidate the role nitrogen uptake utilization pathways durg Magnaporthe oryzae fection， this study，the expression patterns genes associated with rice nitrogen absorption，metabolism utilization signalg pathways were analyzed by real-time RT-qPCR after blast oculation.The results showed that among the 33 genes regulatg nitrogen uptake utilization，22 genes cludg 8 nitrate transporter genes，3 ammonium transporter genes，3 nitrogen assmilation-related genes，5 nitrogen signalg regulatory genes， 3 nitrogenutilization-related genes were significantly up-regulated at 4 hours after fected by a compatible M oryzaerace，which showed no significant changes compared to the control durg the middle to late fection stages （8～96h ）； 28 genes cludg 11 nitrate transporter genes，4 ammonium transporter genes， 5 nitrogen assimilation-related genes，5 nitrogen signalg regulatory genes， 3 nitrogen utilization-related genes were down-regulated upon oculation with an compatible M . oryzae race （4～96h） . These fdgs demonstrated thatthe activation nitrogen absorption，metabolism， utilization signalg pathways played critical roles facilitatg early M.oryzae fection，which could provide references further unravelg the molecular mechanisms nitrogen-duced susceptibility to rice blast.

wordsRice；Rice blast； Nitrogen-duced susceptibility（NIS）；Nitrogen absorption utiliza-tion signalg pathway ； Gene expression

高氮肥施用在促进作物高产的同时也容易引起作物病害的加重，这种现象被称为氮诱导的感病性（nitrogen duced susceptibility，NIS）[1-4]。（剩余12937字）