滇水金凤花距发育相关基因MADS-EJ2 和MADS2的克隆及表达分析

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中图分类号：S681.1：Q781 文献标识号：A 文章编号：1001-4942（2025）09-0001-08

Cloning and Expression Analysis of MADS-EJ2 and MADS2 Genes Related to Spur Development in Impatiens uliginosa

Wang Tianye， Yang Mengqing，Ma Hui， Liang Guangrong， Huang Meijuan， Huang Haiquan （College of Landscape Architecture and Horticulture Sciences，Southuest Forestry University/Researchand Development Center ofLandscape Plants and HorticultureFlowers，Southwest Forestry University，Kunming 65O224，China）

AbstractSEPALLATA（ SEP ）-like，a class E MADS-box transcription factor gene， plays a key role in development of flower organs. In order to investigate the function of SEP -like gene in the spur development of Impatiens uliginosa，the genes MADS-EJ2 and MADS2 were successfully cloned in the present study，named IuMADS-EJ2 and IuMADS2，and both of them had a cDNA sequence length of 762 bp and encoded for 253 amino acids.Bioinformatic analysis demonstrated that IuMADS-EJ2 was a hydrophilic stable protein，and IuMADS2 was a hydrophilic unstable protein，both of them contained MIKC structure and had their sub-familyspecific SEPI and SEP I motifs at C terminus，which were class E genes.Subcelular localization predicted that IuMADS-EJ2 and IuMADS2 were located in nuclei. The amino acid sequences of IuMADS-EJ2 and IuMADS2 genes were both clustered with the homologous gene of Impatiens glandulifera with the similarity of （204号 98.03% and 95.26% respectively. The qRT-PCR analysis indicated that both the two genes expressed at three development periods and two different parts of I . uliginosa spur. IuMADS-EJ2 expressed the highest in the petal spur at the early stage，and IuMADS2 expressed the highest in the limb at the early stage.It was speculated thatthe two genes regulate the development of petal spur，especially at the early stage of petal spur development.The results could provide theoretical bases for the development mechanism analysis of petal spur，the improvement of flower form and the new variety cultivation of Impatiens.

KeyWordsImpatiens uliginosa; Petal spur development ; MADS-EJ2 gene; MADS2 gene; Bioinformatics analysis ； Expression analysis

花距是指在某些植物的花中，其花瓣、萼片等向后或侧面延伸成管状或囊状的结构[1]。（剩余11779字）