基于转录组测序鉴定大豆响应铝毒胁迫的转录因子

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中图分类号：S565.1：Q781 文献标识号：A 文章编号：1001-4942（2025）09-0022-09

Identification of Transcription Factors in Response to Aluminum Toxicity Stress in Soybean Based on Transcriptome Sequencing

Wu Lanhua， Song Yingpei， Kong Keke，Wang Can，Wu Yang， Zhou Huiwen （College of Pharmacy and Life Sciences，Jiujang University/Soybean Research Institute，Jiujiang 3320o5，China）

AbstractIdentification of the transcription factors （TF） in response to aluminum（Al） toxicity stress in soybean is conducive to the analysis of the molecular mechanism of soybean resistant to Al toxicity.In this study，Al-tolerant soybean germplasm Nannong 99-6 （NN99-6）and Al-sensitive soybean germplasm Zhongdou 32（ZD32） were used as materials. After AlC13 treatment， the differential expression TFs （DETFs） were detected by RNA-seq，the functional annotations and protein interaction analysis were performed，and the expresion levels of the key TF genes detected by qRT-PCR.The results showed that 387 DETFs were obtained in response to Al toxicity stress，and 83of which showed diffrential expression patterns between Al-tolerant and Al-sensitive soybean germplasms.Five key DETFs interacted with each other， containing NAC6， WRKY58，WRKY30，WRKY57 and I1MEN3_SOYBN. Compared with 0h ， the five DETFs genes were significantly up-regulated at 6，12，24，48 h and 72h under Al toxicity stress，and the expression level of WRKY58，WRKY30 and WRKY57 increased with the extension of treatment time.In conclusion，this study identified that five key DETFs might play important regulatory roles in soybeanresponse to Al toxicity stress. The result could provide a theoretical support for verifying the function of Al resistance related genes in soy bean.

KeyWordsSoybean; Al toxicity stress； Diferential expression TF genes； Protein interaction; NAC;WRKY

大豆是世界上重要的油料作物，为人类提供了大量的食用油和高蛋白原料。（剩余12669字）