海岛棉R2R3-MYB基因家族的鉴定及其在黄萎病胁迫下的表达分析

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中图分类号：S562：Q786 文献标识号：A 文章编号：1001-4942（2025）06-0001-14

AbstractR2R3-MYB transcription factors play crucial roles in the process of plants responding to stress. In this study，based on the genomic data of Gosspium barbadense，the members of the R2R3-MYB gene family in G .barbadense were systematically identified. Bioinformatics methods were used to analyze the phyletic evolution，structure，physicochemical properties，and cis-acting elements of the promoter of the R2R3-MYB gene family in G . barbadense. Five candidate genes for Verticillium wilt resistance were screened out using transcriptome expression profiles，and the expression patterns of each candidate gene under the induction of Verticillium wilt，salicylic acid （SA）and methyl jasmonate （MeJA） were analyzed by real-time fluorescence quantitative PCR. The results showed that there were 98 R2R3-MYB genes in the genome of G .barbadense，which distributed on 25 chromosomes.The phylogenetic tree analysis indicated that the members of the R2R3-MYB gene family in G. barbadense could be divided into three large subgroups，and they were relatively conserved in evolution.Promoter analysis revealed multiple cis-acting elements related to plant hormonesand stress responses.The expression patern analysis after Verticillium wilt stress showed that all the R2R3-MYB genes in G barbadense had different degrees of diferential expression after Verticillium wilt stress.Five candidate genes for Verticillium wilt resistance in G. barbadense （GbMYB-21，GbMYB-34，GbMYB-39， GbMYB-76，and GbMYB83）were screened out，which were all induced by Verticillum wilt stress，SA and MeJA.In conclusion，this study demonstrated that the R2R3-MYB gene family in G. barbadense played an important role in coton disease resistance，which could provide a theoretical basis for further research on the functions of R2R3-MYB genes in G. barbadense and the molecular mechanism of cotton resistance to Verticillium wilt.

KeywordsGossypium barbadense； R2R3-MYB gene family； Bioinformatics analysis； Verticillium wiltstress ； Expression analysis

棉花是我国重要的经济作物，在纺织、国防、医药和汽车等领域有着重要的用途。（剩余14606字）