铬胁迫对苹果组培苗生理特性的影响

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中图分类号：S661.1 文献标志码：A 文章编号：1002-2910（2025）03-0018-05

Effect of chromium stress on the physiological characteristics of apple tissue culture seedlings

XUZhaoru1，HEDongyu²，CHEN Ziwen²，LILijiao²，LILing²，CHENXiude2，3*

（1.CollegeofFutureTechnology（QiluColege），handongAgriculturalUniversity，Tai'an，handong2018，

China;2.CollgeofHorticultureScienceandEngineering，handongAgriculturalUniversity，Tai'an，andong

，China;3.AppleTechnology InnovationCenterofShandongProvince，Tai'an，Shandong，China） Abstract： The effects of different concentrations of potassium dichromate on the physiological characteristics of P1 tissue cultured seedlings of apple rootstock were studied.The subculture medium was treated with potassium dichromate at four concentrations of25，50，100 and 200mgL with 0mg/L asthe control.Physiological indicators were measured every 7 days after treatment.The results showed that tissue cultured seedlings were negatively affected by 25～200mg/L chromium treatment，which was positively correlated with the treatment concentration and varied with the extension of treatment time until it decreased.The chlorophyllcontent in seedlings decreases with

increasing treatment concentration;After the same treatment， the content began to decrease on the 21st day，and the greater the chromium treatment concentration，the greater the decrease； The activities of soluble sugars，antioxidant enzymes SOD，POD and CAT increased with the increase of chromium treatment concentration，and showed a trend of first increasing and then decreasing with the prolongation ofchromium treatment time.After 7days of treatment，low concentrations of 25and 50mg/L potassium dichromate had little effect on the seedlings，and the enhancement of antioxidant enzymeactivity was relatively small.At14or21 daysof treatment，the activities of antioxidant enzymes SOD，POD and CAT in the seedlings treated with high concentrations of 100 and 200mg/L decreased，and the chlorophyll content decreased.The seedlings were subjected to chromium stress toxicity.

Key words： chromium; stress; apple tissue culture seedlings; physiological characteristics

铬（Cr）属于重金属元素，是人体必需的微量营养元素之一，一般认为二价铬是无害的，三价铬是动物体必需的活性元素，六价铬是环境污染物之一。（剩余5580字）