不同膻味水平的龙陵黄山羊肝组织转录组SNP分析

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摘要：为加强山羊肉类的质量，探究膻味相关差异基因，对3个品种共14只羊的总膻味值进行显著性差异检验发现，高膻味水平（总膻味值大于771.845）和低膻味水平（总膻味值低于771.845）龙陵黄山羊总膻味值差异显著（P

关键词：龙陵黄山羊;肝脏;SNP;GO富集;KEGG富集

中图分类号：S826.8+9         文献标识码：A

文章编号：0439-8114（2022）04-0154-06

DOI：10.14088/j.cnki.issn0439-8114.2022.04.031 开放科学（资源服务）标识码（OSID）：

SNP analysis on liver transcriptome of Longling yellow goat with different odor levels

MA Lin， YANG Ming， WANG Xuan-yi， YE Shao-hui

（College of Animal Science and Tecnology，Yunnan Agricultural University，Kunming  650201，China）

Abstract： In order to enhance the quality of goat meat and explore the difference genes related to the odor， the total of 14 sheep of three breeds were tested for the significance difference of the total odor value. It was found that there was significant difference （P< 0.05） between Longling yellow goat with high（total odor value greater than 771.845） and low（total odor value less than 771.845） levels. Transcriptome sequencing of liver tissues of Longling yellow goats with high and low odor levels was carried out， and SNP analysis was carried out on the obtained data， and SNP genes were analyzed by GO enrichment and KEGG enrichment. The results showed that there were 411 973 SNPs peculiar to Longling yellow goats with high odor level and 444 638 SNPs peculiar to Longling yellow goats with low odor level， and the common SNPs of two were 856 611. GO enrichment analysis showed that SNP gene was enriched in biological process and cell composition. KEGG enrichment analysis showed that SNP gene was significantly enriched in phosphatidylinositol signaling system， complement and coagulation cascade， insulin resistance， splice， autophagy-animal， cofactor biosynthesis， protein processing in endoplasmic reticulum， endocytosis and AMPK signaling pathway. Analysis of SNPs of six genes in fatty acid biosynthesis pathway showed that seven SNPs were located in synonymous coding regions， and two SNPs caused amino acid changes.

Key words： Longling yellow goat; liver; SNP; GO enrichment; KEGG enrichment

羊肉在世界各地广泛食用，但由于其膻味，在许多亚洲国家并不受欢迎。（剩余8806字）