基于A u@Ag 纳米粒子的氨基酸表面增强拉曼散射检测

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中图分类号：0657.37文献标志码：A

Surface-enhanced Raman scattering detection of amino acids based on Au @ Ag nanoparticles

CHANGMin，LIU Huifang，SHI Yueyan，ZHANG Jiachang，LI Shibo，HE Chen （School of Optical-Electrical and Computer Engineering， University of Shanghai for Science and Technology， Shanghai 200093，China）

Abstract： Surface-enhanced Raman scattering （SERS） is widely employed for amino acids quantitative analysis due to its high-sensitivity molecular feature extraction and multiplex detection advantage. Stable and highly sensitive SERS substrates are crucial for achieving precise detection. Gold core silver shell nanoparticles （Au@Ag NPs） combine the chemical stability of gold nanoparticles with the high SERS enhancement effect of silver nanoparticles.They enable the screening and detection of seven amino acids， including phenylalanine and alanine. Except for isoleucine and glycine， which exhibited relatively lower sensitivity，the detection limits for the remaining amino acids reached 1×10-4mol/L . A linear correlation existed between concentration and SERS signal intensity， with correlation coefficients exceeding 0.94. This study provides a reference method for simultaneous quantitative detection of multiple amino acids on a single substrate.

Keywords： amino acid; surface-enhanced Raman scatering; Au@Ag NPs; quantitative detection

引言

氨基酸是构成蛋白质的基本单元，在人体生命活动中发挥重要作用，因此对必需氨基酸需保证日常摄人。（剩余9078字）