基于微流控芯片的肿瘤细胞球三维培养及药物筛选研究

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中图分类号：TB39文献标志码：A

Research on three-dimensional culture and drug screening of tumor cell spheres based on microfluidic chips

XUE Zhiwei， ZHENG Lulu （School of Optical-Electrical and Computer Engineering， University of Shanghai for Science and Technology，Shanghai 20oo93，China）

Abstract： Three-dimensional culture of tumor cell spheres can truly simulate the growth environment of tumor cels in vivo， and thus be more suitable for drug screening and other related research. Combined with microfluidic technology， there are more ways to implement threedimensional cell culture technology. In this study，a microfluidic chip for high-throughput threedimensional tumor cell spheres culture was designed to enable rapid cultivation and drug screening of tumor cell spheres based on microcavity arrays. Compared to the current mainstream threedimensional culture that takes 4 days， microfluidic chips only require 15h to form tumor spheres. It significantly saves cultivation time. The reliability of three-dimensional culture in the chip was verified by tumor growth morphology monitoring and fluorescence imaging technology.

Meanwhile， this research realized multi-drug rapid screening based on chip， presented good gradient dependence and verified the feasibility of drug screening in chip. This high-throughput microfluidic chip can be applied in multiple research fields such as oncology， providing a new technological means and experimental platform for achieving rapid drug screening in tumors.

Keywords： microfluidic chip; three-dimensional culture; drug screening

引言

在世界范围内，癌症患者的发病和死亡数量持续增长。（剩余7937字）