海水叶绿素a传感器荧光性能评估方法

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A standardized method for evaluating the fluorescence performance of

seawater chlorophyll-a sensors

WANG Aijun，WANG Cong， SHI Chaoying， ZHAO Hong （Technology Innovation Center for Marine Metrology and Instruments Test， MNR，National Center of Ocean Standards and Metrology，Tianjin ，China）

Abstract：Seawater chlorophyl-asensorsare essential tools for marineecological monitoring，enabling thedetectionof spatialand temporal variations inchlorophylla concentration.However，thesesensorsaresusceptible to measurement drift，whichcancompromisedatareliability.Thisstudyproposesametrologicalapproachforevaluating sensor performance using fluorescein sodiumasa reference standard.Sensor performance was assssed in terms of linear responserange，accuracy，precision，and stabityResults indicatedastrong positive corelationbetween fluorescence intensityandfluoresceinconcentration.Basedonthefittedcalibrationcurve，indicationerorand standarddeviation were calculated.The linearresponserange wasdeterminedbycontroling thecorrelationcoeficient，whereasstability was

assessed through repeated measurements over diffrent time periods.Within the linear range of 0 to 200μg/L ，the maximum measurement error was ⩽2.00μg/L ，and the relative standard deviation was <0.20% .The sensor exhibited consistentperformance from2021to2022.Maintaining consistent pipetingaccuracywasidentifiedasacriticalfactorfor ensuringmeasurement reliability.

Key words ： seawater chlorophyll-a senaor; fluorescence standard material； evaluation method； pipete accuracy

海洋中叶绿素a质量浓度及其时间变化可以反映海水中浮游植物的丰度及其变化规律，在一定程度上反映水体中藻类数量和水质状况[1-2]，是海洋生态监测的常规监测项目之一，在研究浮游植物群落、海洋生态环境监测、赤潮防灾减灾等方面起着重要的作用。（剩余6498字）