杉木不同世代育种群体遗传多样性回溯分析与核心种质构建

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中图分类号： S722.3+1 文献标志码：A

Abstract：【Objective】Toanalyze thegeneticdiversityandgenetic structureofdiferentgenerationsofChinese firbreeding populations，econstructthegeneticpedigreeofbreedingparentsfromeachgenerationtoguidetheselectionandmatingofbreeding parents，andestablishacoregermplasmofChinesefir.【Method】Atotalof388Chinesefrbredingclonalparentsfromdierent generations（74fromtheinitialgeneration，70fromthefirsgeneration，107fromthesecondgeneration，andl37fromthethird generation）wereusedasmaterials.GeneticdatawereanalyzedbasedonESTSSR，andtheoverallevelofbreedingpopulations was reflectedbythepolymorpismandgeneticdiferentiationparametersof16ociGeneticdiversityparameters，geneticvariationand geneticdiffeentiationecentsreusdtoaaletgeneticdivesitylevelsofCineefrreingpoplatiosacodient generations.Clustering analysisand population structure analysis wereconductedtodetermine population groupings and genetic pedigreesofbreeding populations.Coregermplasmwasseletedbasedontheprincipleofmaximizingalleles.【Result】1）Atotal of 640 polymorphiclociwereamplifiedwith16primerpairs，withanaverageof40allelesperlocus.The meanefectivenumberof aleleswas16.25，theShanonindexwas2.942，themeanpolymorphisminformationcontent（C）was0.942，andthemeanfation indexwas0.97indicatinghighpolymorpismamongpopulations.TeoverallgeneticdiversityofteCinesefrredngoplations washighethantatofteinitialgeneratio，withecondandthidgeneratiosehbitinghigherdiversitytantheovealevel; 2）Thefourbreedingpopulatnseredividedintoteediferetgroupsithindividualsgroupedaccordgtomateralcaracteistic andgeneticrelationships;3）Atotalof127coregermplasmswereselected，andtheconstructedcoregermplasmbankretained 100% of thealeles.Thefectivenessoftecoregermplasmbankwasverifedthroughageneticparametertest.【Conclusion】Tedieent generationsofesefireedingpoplatioseiedggeticdversitydlowgneticderean，dicatigtatdace generationbreedingefectivelymaintains geneticvariation withinpopulations.Thereconstructedpedigreemapofbreedingparents providesasientificbasisforteatoalseectionndrossbreedingofeliteparentsMoreoverthidentifedorcolectietains 100% oftheallelicdiversityoftheorigialgermplasmandshowsstrongrepresentativeness，ayingasolidgeneticfoundationforthe futureelectioofsuperiorarieties，fentutilationofgerplasesoues，andsustaiabledevelopmentofreedingolations. Keywords：Cunninghamia lanceolata;breedingpopulations; geneticdiversity;geneticstructure;corecollection

杉木Cunninghamialanceolata属于柏科杉木属单种常绿大乔木，是我国长江流域以及秦岭以南地区栽培最广、生长快且经济价值高的针叶用材树种。（剩余15945字）