山茶WRKY基因家族鉴定及其在花色形成中的表达分析
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中图分类号：S794.9 文献标志码：A 文章编号：1673-923X（2025）07-0188-09
Identification and expression analysis of WRKY gene family in flower color formation in Camellia japonica
SONGZhixin1²，FANMenglong1，JIANGHong1，ZHANGYing1，LIXinlei1 （1.ResearchInstituteofbtropicalForestryineseAcademyofForestry，Hangzhou340o，Zhejang，ina 2.Nanjing ForestryUniversity，Nanjing21oo37，Jiangsu，China）
Abstract：【Objective】The WRKYgene familyplays animportantrolein theregulation of plant growthand development.Camelia japonicaisanimportantoamentalplantwithichfowercolors.ItisofgreatsignificancetoidentifyandanalyzetheWRKYgene familymembers inCamelia japonica petals withdiferentcolors.【Method】Basedonthe whole genome dataofCameliajaponica， theWRKYgenefamilywasidentifedandanalyzedbybioinformaticsmethods.Basedonthetranscriptomedatarelatedtocamellia flowercolor，teexpressionpateofCjWKYsindifentcoloedamellapealsasaalyed【Result】esultsindcatedtat 94 CjWRKYgenes were identified in the whole genomeand named CjWRKY1-CjWRKY94.The phylogenetic tree dividedthe strains intothregos（I，I）adgopIIwafurthvieditfiveubous（a，I-b-c，I-I-e）.ob contained twoWRKYdomains，whilegroupI andIImemberscontainedonlyone WRKYdomai.Atotalof10conservedmotifsand 5conserveddomainswereidentifed.TheCjWRKYsmembersinthesamesubgrouphadsimilarconservedmotifs，conserveddomains and genestructures.The CjWRKYs promoterregioncontained 38 elements forlightresponse，stressresponseandhormonerespose， includingMYBbindingsiteforregulatingfavonoidbiosynthesis.94CjWRKYswereunevenlydisrbutedon15chromosomes，and there were13 pairsoftandemduplication events.Collnearityanalysisshowed thatthere were72duplication events in94CjWRKYs genes，and the Ka/Ks valueof the duplication genes was less than1.There were 116 colinear gene pairs with Arabidopsis WRKY gene family.Amongthem，32CjWRKYs wereup-regulatedand6CjWRKYsweredown-regulated withthedeepeningofflowercolorof Cameliajaponica，whichsugestedthat CjWRKYs plaedanimportantroleinteregulationofanthocyanisyntesis.Itisspeclated thatCjWRKY48，CjWRKY53andCjWRKY6 positivelyregulateanthocyaninsynthesisbyinteracting withMYBtranscriptionfactors， therebyaffectingflowercolorofCameliajaponica.【Conclusion】94CjWRKYsgeneswereidentifedinthewholeCamellajaponica genome，andmostCjWRKYsareinvolvedinanthocyaninsynthesisandplayanimportantroleincolorformationofCameliajaponica.
Keywords：Camellia japonica;WRKYgene family;flowercolor;expressionanalysis
WRKY基因家族是高等植物中最大的转录因子家族之一，参与植物中大部分生理代谢过程，包括调控生长发育、参与胁迫响应以及次生代谢物生物合成等[]。（剩余17502字）