杜仲叶片不同发育时期转录组及绿原酸相关基因分析

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中图分类号：S792.99 文献标志码：A 文章编号：1673-923X（2025）07-0174-14

Analysisof transcriptomeand chlorogenicacid related genesin Eucommia ulmoides leaves at different developmental stages

WANGSiran1，LIHui，Aricha’，JINMulan²，QINGJun1，BAIYu’e1 （1.CollegeofForestryInnerMongoliaAgriculturalUnversity，Hohhot Ooo19，Inner Mongolia，China; 2.UlanqabForestryand GrassandMonitoring andPlanning Station，UlanQab O120o，Inner Mongolia，China）

Abstract：【Objective】Chlorogenicacid isanindex componentforthequalityevaluationofEucommiaulmoides leaves，whichhas variousmeiciallusschtbactealdtamtorytiodanttacrdtptitieatio andexcaationofmetabolicpathwaysandgnesinvolvedinregulatingchlorogenicacidbiosynthesis inEucommiaulmoidesleaves willprovidefurtherevidenceforanalysisofthemolecularmchanismofchlorogenicacidformationinEucommiaulmoidesleaves. 【Method】Eucommialmoidesleavesrecectedfromotou，Iergoliautonomousgion，inpril，Julydctober 2023at diferetdevelopmenalperods，andwereclaifedaccordingtotecharacterationas：Aprilfreshlyunfoledyoungleaves; Julymatureleaves，withlighteathery;andctoberfullymatureaves，withthdarkestcoloroftelavesandompleteleathrye chlorogenicacidcontentofEucommiaulmoidesleavesatdiferentperiodswasdeterminedbyig-performanceliquidchromatography （HPLC），andthediferetiallexpressedgenesereidentifdbyrasiptomesequencing.【Result】Teloogniccidctet ofEucommiaulmoidesleavesrangesfromApriltoJulyandthentoOctober，showsanincreasingandthendecreasing trend withleaf development.Transcriptome sequencing yielded 58.06 Gbof clean data，enablingthe identification of11 689 genes.The numberof down-regulatedexpressdgnesisigherthanteumberofupregatedexpresedgenesinteompasonofsTTsand

T2vsT3hreegroups.KEGGenrichmentanalysisandGOenrichmentanalysis，bothofwhichdisplaychlorogenicacidsynthsis-related enrichments.FurtemoreKmeanstredexpreionividsteensinto0modles，inwhichtrendsofgeneexpressinlass4， Class8adClassareconsstentwithtrendsoforogenicacidotetadtwozsandegensareidentifdfroClass4 Class8andClass10tatarerelatedtoclorogenicacidbiosythsis.Tresultsofal-tiefuoreseequanitativePCR（-CR） are inaccordance withRNA-seqoutcomes.【Conclusion】ThechlorogenicacidcontentintheEucommiaulmoides leavesshowsa trend of increasing and then decreasing with the development of the leaves.

Keywords：Eucommiulmodesorogeniccid;trsiptoe;difrentiallyexpreedneDEGs）;iofoatiais

杜仲Eucommiaulmoides落叶乔木，为杜仲科杜仲属植物，是我国名贵药材之一，亦是药食同源植物。（剩余16746字）