荧光寿命成像技术及其在生物医学领域的应用

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关键词：荧光寿命成像技术；频域法；时域法；生物医学中图分类号：TN247；0657.3 文献标识码：ADOI：10.37188/CJL.20240240 CSTR：32170.14.CJL.20240240

Fluorescence Lifetime Imaging Technology and ItsApplicationsin Biomedical Science

YANWanlu1，SUWeitaol*，CHENDaqin² （1.SchoolofScience，HangzhouDianzi University，Hangzhou31oooo，China; 2.School ofPhysicsand Energy，FujianNormal University，Fuzhou 35oooo，China） *CorrespondingAuthor，E-mail：suweitao@hdu.edu.cn

Abstract：Fluorescence lifetime imaging is atechnique widelyused in various fields.Ithas high sensitivity，high timeresolutionandspatialresolution.Moreover，italsocan beused toquantitatively study thesamplewithoutthe influence of excited laser and environmental factors.Because of the above advantages，fluorescence lifetime imaging hadbeenwidelyused inbiomedicalfield.Fluorescencelifetimeimaging techniquecanbedivided into frequencydomain and time domain routines.Frequency domain routinecanbe divided into heterodyne method and homodyne methodaccording tothedifference frequency characteristics.There arethree major methods torealize time-domain routine，i.e.gating method，time-correlated single photon counting method（TCSPC）and scanning camera measurement method.This work introduces the basic principles of aforementioned fluorescence lifetime imaging methods. Wethenreview the recent application offluorescence lifetime imaging in biological sciencesandthe clinic study of tumorcancercell，and further give itsperspectives.

Keywords：fluorescencelifetimeimaging technology；frequencydomainmethod;timedomainmethod;biomedicalscience

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