海洋链霉菌OUCMDZ-2582产生的吲哚咔唑生物碱及其抗菌活性

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中图分类号：R978 文献标志码：A

Indolocarbazole alkaloids from marine-derived Streptomyces sp. OUCMDZ-2582 and their antibacterial activity

Xu Guangjin1， Wen Qingyun1，Bu Shutian1， Chen Linmeng1， and Zhu Weiming1.2 （1KeyLaboratoryofarinerugs，inistryEducationofhina，hoolofedicineandPamacyOceanUiversityofa

AbstractObjective To investigate the indolocarbazole alkaloids produced by marine-derived Streptomyces sp. OUCMDZ-2582 and their antibacterial activity. MethodsA1 liquid culture medium was used to ferment the Streptomyces strain.The fermentation broth was extracted by ethyl acetate （EtOAc）. Guided by the characteristic ultravioletabsorptionof indolecarbazole，the indolecarbazole alkaloids inthe fermentation products were isolated by column chromatographyover positive and reverse phase silica gel，Sephadex LH-2O，aswellassemi-preparative high-performance liquid chromatography （HPLC） to give pure compounds. The structures of the compounds were elucidated through theanalysisofLC-MS，NMR，andoptical rotation，etc.The antibacterial activity was assyed by microbroth dilutionmethod.ResultsNine indolocarbazole alkaloids were purified and identified as staurosporine （1），3'-N-acetsturooine （2），3--burospie（3），A （4），trtocboleC（5），b， 3'-N-acetylholyrineA（7），3'-N-acetyl-3-ydroxyholyrineA（8），andK25c（9），respectively.Teantibacterialaciviie for compounds 2and 3 against Vibrio parahaemolyticus and compound 3 against Vibrio alginolyticus were observed， each at a minimum inhibitory concentration（MIC） of 32μg/mL . Conclusion Streptomyces sp.OUCMDZ-2582， isolated from the Yellow River Delta，is a prolific producer of indolocarbazole alkaloids. Compounds 2 and 3 displayedantibacterial activities.

Key WordsMarine Streptomyces; Indolocarbazole alkaloids; Structural elucidation; Anti-bacterial activity

海洋几乎占据了地球表面的3/4，海洋环境的特殊性造就了海洋生物特殊代谢途径和适应机制，海洋天然产物独特而多样的化学结构使其具有显著的生物活性1，其中海洋微生物更是海洋天然产物的主要生产者[2-3]。（剩余14714字）