噬菌体作为载体传递CRISPR系统的应用研究进展

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中图分类号：R378 文献标志码：A

Advances in the application of phage as a carrier for the delivery of CRISPR system

Chen Xi1 ，WenYuanxi²，KanBiao1²，andFanFenxia1 （1NationalIstituteforCommuicableiseaseontrolandevetio，CineseCenterforseaseControlandPrevetioeiing 102206a;2DepartmentofidemiologydHealthatisticshoolofublicHalthadongUiversitJ）

AbstractBacteriophages，as viruses capable of cracking bacteria，are the most abundant and diverse biological entities on earth.Phage research hasadvanced many fields of biology and molecular biology.Based on the global health issue ofbacterialresistance and health threats caused by excessiveuse and abuseofantibiotics，bacteriophages as potential therapies have attracted atentionagain.Phage therapy has itsadvantages，but also has some limitations. Using genetic enginering and synthetic biology technology， the CRISPR system and phage genome are integrated together，andthephageisusedasadeliverycarrier.Finalyadual-targetedelminationstrategyisformed，inwhich phage accurately targets specific pathogens and CRISPR accurately targets specific genes.Thisarticle introduced the relevant modification strategies and application reports on the recombinant integration of phage and CRISPR sequences，discussed theadvantages and disadvantages of phage as a delivery vector，and proposed theuse of phageCRISPR.

Key wordsTemperate phage; Virulent phage; Lysis; Modification; CRISPR-Cas; Targeted therapy

自噬菌体被发现之初，其潜在治疗与预防传染病的应用价值便备受关注。（剩余16524字）