短梗木霉与芽孢杆菌固体共培养物WP剂型研制

打开文本图片集

中图分类号：TQ450.6 文献标志码：A文章编号：1673-9868（2025）05-0090-13

Preparation of Wettable Powder for Solid Co-Culture of Trichoderma breve LNoo6 and Bacillus velezensis ZJ

WU Yiping1， WANG Jingwen¹， ZHANG Yaxuan1， LIU Tong1，²

1.Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests of Ministry of Education/School of Tropical Agriculture and Forestry，Hainan University，Danzhou Hainan 571737，China; 2.School of Breeding and Multiplication，Hainan University，Sanya Hainan 572020，China

ibstract： Trichoderma breue LNoo6，which has a growth-promoting effect and disease prevention effect on plants，and Bacillus velezensis ZJ were used as functional strains to prepare a wettable powder （WP） by solid co-culture，grinding，and mixing with carriers and dispersants. The effect of its application on rice sheath blight was measured. The results showed that the number of spores of Trichoderma in the co-culture products was 7.51×109CFU/g ， and the number of bacterium was 9.22×109 CFU/g，and the optimal formula of wettable powder was as follows： 20% product of co-culture， 66.6% hydrated silica， 5% Tween-20， 8% sodium carboxymethylcellulose and 0.4% lecithin. The wetting time of the formula was 41.72s ， the suspension rate was 87.79% ，the pH value was 7.11，the pass rate of 20O mesh sieve was 95.77% ，the number of live spores of Trichoderma was 1.45×109CFU/g ， and the number of live bacterium was 1.78×109 CFU/g. The foaming property is 14mL ，which meets the requirements of the national standard. There was no significant difference in control effect on rice sheath blight between WP （65.12% ） and control agent Jinggangmycin （72.09% ）. The germination test showed that the plant height and root length of WP treated rice were increased by 18.67% and 10.47% compared with the control， respectively. （20 T . breue LNoo6-B. velezensis ZJ WP provides a new way for green and sustainable prevention and control of rice sheath blight.

Key words： Trichoderma breoe ; Bacillus velezensis ； compatible strains; co-culture； wetble powder

水稻纹枯病是由立枯丝核菌（Rhizoctonia soani）引起的土传真菌病害，在秧苗期至穗期均可发生，可造成严重减产。（剩余13701字）