埃塞俄比亚芥菜中BcMYB28 基因调控硫苷代谢分析

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中图分类号：S635.9 文献标志码：A文章编号：1673-9868（2025）05-0038-15

DOI：10.13718/j. cnki. xdzk. 2025. 05.004

BcMYB28 Involved in the Regulation of Glucosinolate Metabolism in Brassica carinata L.

WANG Yanhua1， SHAO Weixing²， FANG Shal， AN Dongyan

1.Colege of Horticultureand Landscape Architecture，Southwest University/Key Laboratoryof Agricultural Biosafetyand Green Production of Upper Yangtze River Ministry of Education，Chongqing 400715，China; 2. Chongqing Academy of Agricultural Sciences，Chongqing 401329，China

Abstract： Ethiopian mustard （Brassica carinata ，BBCC 2n=34 ） has many excellent agronomic traits，including vigorous growth in the seedling stage， drought tolerance，broad-spectrum disease resistance， cracking resistance，etc. However，the utilization of the above-mentioned excellnt features is limited by its high glucosinolate characteristics. To reveal the potential function of the BcMYB28 gene in the biosynthetic pathway of glucosinolates in Ethiopian mustard and find the key regulatory genes of glucosinolate metabolism. In this study，the BcMYB28 gene was cloned from Ethiopian mustard，and six homologous genes（BcMYB28.1-BcMYB28.6） were obtained through comparison analysis. The similarity between them and the MYB28 gene family members of Brassica crops is 74.6%-84.9% . CRISPR/Cas9 was used to knock out BcMYB28 to obtain a T1 generation of edited line. The leaves of the edited line showed phenotypic changes such as loss of wax layer. RT-qPCR showed that BcMYB28 gene expression was significantly reduced.1 843 species of secondary metabolites were identified using UHPLC-MS technology. Compared with wild type，there were 247 up-regulated and l32 down-regulated metabolites in the edited lines. The glycine，serine and threonine metabolic pathways were significantly enriched. Through transcriptome sequencing，using B . oleracea and B . napus as the reference species，5 495 and 5 104 differentially expressed genes （DEGs）were obtained，respectively. GO and KEGG enrichment analysis revealed 20 GO terms were significantly enriched. Cluster analysis was performed on DEGs，and 10 key genes related to glucosinolate synthesis were identified.

Key words： Brassica carinata ; BcMYB28 ;transcriptome; metabolome

埃塞俄比亚芥菜属于十字花科芸臺属作物，由黑芥和甘蓝天然杂交，自然加倍进化而来[1-3]。（剩余15907字）