Box-Behnken响应面法优化高良姜中山奈酚提取工艺及其抗氧化活性研究

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关键词：高良姜；山奈酚；高效液相色谱法；提取工艺；抗氧化活性

中图分类号：R282.5 文献标志码：A 文章编号：1007-3213（2025）08-2034－09

DOI：10.13359/j.cnki. gzxbtcm.2025.08.030

Abstract：Objective To determine the optimal extraction process for kaempferol from Alpinia oficinarum Hance and to investigate its antioxidantactivity.Methods Basedon single-factor experimental data，this study employed the Box-Behnken central composite design and response surface methodology（RSM），combined with highperformance liquid chromatography（HPLC），to plot response surface and contour maps using kaempferol content as the response value.The optimal parameters for ethanol concentration，solid-liquid ratio，extraction time，and extraction temperature were selected.The antioxidant activity of kaempferol was comprehensively evaluated by calculating the radical scavenging rate using the DPPH radical scavenging assys.Results The optimal extraction conditions were determined to be an ethanol concentration of 84% ，asolid-liquid ratio of 1：80 ，an extraction time of 2 hours，and an extraction temperature of 82C . Under these conditions，the kaempferol content extracted from Alpinia officinarum Hance reached 0.03416% . In the DPPH radical scavenging assay， the extract showed a scavenging rate of 91.20% at a concentration of 1 mg/mL. Conclusion The optimized extraction process for kaempferol from Alpinia offcinarum Hance was obtained using the Box-Behnken response surface design method，and this process is practical and feasible.The DPPHradical scavenging assaydemonstrated thatkaempferolfrom Alpinia offcinarum Hance possesses antioxidant activity.

Keywords：Alpinia oficinarum Hance；kaempferol；high-performance liquid chromatography（HPLC）； extraction process；antioxidant activity

高良姜（AlpiniaofficinarumHance），又称高凉姜、良姜，为姜科植物，主要分布于中国南部和东南亚等地。（剩余10152字）