花生两种不同分化类型愈伤组织的差异表达基因分析

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Abstract： Tissue culture is a crucial step in the genetic transformation of peanut （Arachis hypogaea L.）. However，the differentiation of peanut callus is restricted by genotype. In this study，Luohua 22，a cultivar with strong callus differentiation ability，was screened from 9 germplasm resources through establishing a peanut genetic transformation system.Transcription sequencing analysis were carred out using T1callus（embryogenic calus prone to differentite into seedlings），T2 callus （non-embryogenic callus difficult to diferentiate into sedlings）during the differentiation process ofLuohua 22，and callus atOday of differentiation was set as control （CK）.Compared with CK，there were 1792 and 868 diferentially expressed genes（DEGs）in T1 and T2 types of calus，respectively.The GO term enrichment analysis showed that the DEGs in T1 type of calls were mainly enriched in the terms of meristematic organization and stem cellpopulation maintenance，while those in T2 type of callus were mainly enriched in the terms of phenylpropane biosynthesis and metabolic term.Protein family analysis showed that DEGs in T1 and T2 types of calls contained 2459 protein-coding genes，among which the cytochrome P450 family was significantly enriched.By protein-protein interaction（PPI） network analysis，five key genes AhAE3ZZG，AhP17M1H，AhA6R79F，AhZFZ3ZQ and AhHMN99B，were mined，which might play important roles in promoting peanut callus differentiation.The results of this study would provide a scientific basis for furhter exploring the key genes afcting the differentiation process of peanut cotyledon node calus，and the subsequent analysis of the molecular mechanism of peanut callus differentiation to form regenerated plants.

Key words： peanut; cotyledon node;callus differentiation; RNA-Seq ;protein genes

花生（ArachishypogaeaL.）是重要的油料和蛋白质来源作物，广泛用于饲料、工业等领域[1]。（剩余20911字）