大豆CMS恢复基因Rf2功能性分子标记开发及恢复系新种质创制

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Abstract：The composition quantity Restorer--fertility genes contained in the restorer line directly determine the strength the restoration ability，thereby affecting the fertility stabilityhybrid soybeans bred through the "three line" method. The RfI gene is widely present in soybean cytoplasmic male sterile restorer lines，while Rf2 ， as a newly discovered strong Restorer--fertility gene，only exists in a very small number restorer lines.To this end，this study developed functional molecular marker for the Rf2 gene used conventional hybrid breeding combined with molecular marker-asisted breeding techniques to aggregate the Restorer--fertilitygenes RfI Rf2 ，creating a new germplasm soybean cytoplasmicmale sterile restorer lines with strong restoration ability excellent agronomic traits.The results indicate that the combination Rfl Rf2 molecular markers can quickly accurately screen target Restorer--fertility gene types， significantly improving the breeding efciency new germplasm gene aggregation restorer lines.At the same time，the newly created restorer germplasm showed excellent performance in agronomic traits such as pod number per plant，grain number， 100-seed weight. This study provides excelent new germplasm for the breeding "three line" hybrid soybean， also provides technical support for the efcient breeding more strong restorer lines in the future.

Words： soybean;cytoplasmic male sterility; restorer line;sterile line;molecular marker-assisted selection

大豆［Glycinemax（L.）Merr.]是豆科大豆属一年生草本植物，是重要的粮油饲兼用作物，在中国已有5000多年种植历史。（剩余12823字）