光热剂Cy7-TCF对人胰腺癌细胞氧化应激及凋亡的影响

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[中图分类号] R735.9;R454 [文献标志码] A

Effect of the photothermal agent Cy7-TCF on oxidative stress and apoptosis in human pancreatic cancer celsJIANG Wanyan， JING Xue， ZHOU Xianfeng，TIAN Zibin（Department of Gastroenterology，The Affiated Hospital of Qingdao University，Qingdao 266oo3，China）

[ABSTRACT]ObjectiveTo investigate the efect ofthephotothermal agent Cy7-TCFonoxidative stressand apoptosis in human pancreaticcancercels.MethodsMiaPaCa-2cellswere divided intocontrol group，Cy7-TCF group（treated with Cy7- TCF alone），and Cy7-TCF + laser group （treated with Cy7-TCF followed by 808nm laser irradiation）. CCK-8 assay was used to measurecellviabilityunderdiferentconcentratiosofCy-TCFwithorwithoutlaseriradiation；fluorescecemicroscopywasused toobservetheuptakeofCy7-TCFbycels，andconfocal microscopycombined withalysosomalprobewas usedtoobserve thesubcelular localizationofCy7-TCF；the DCFH-DA fluorescent probewasused tomeasurethelevelof reactiveoxygenspecies（ROS） in cels；flowcytometrywasused tomeasurecellapoptosisrate.ResultsCCK-8assayshowedthatunderlaserirrdiatio，el viability significantly decreased with the increasing concentrationof Cy7-TCF in aconcentration-dependent manner（ P<0.05 ）. Fluorescence microscopyshowed that Cy7-TCF was efectivelytakenupby Mia PaCa-2cels，andconfocal microscopy showed that Cy7-TCF was mainly localized within lysosomes. DCFH-DA probe detection showed that under 808nm laser irradiation，Cy7-TCF significantly increased the level of ROS in Mia PaCa-2 cells ⋅q=19.07，16.84，P<0.05） . Flow cytometry showed that the Cy7- TCF+laser group had a significantly higher apoptosis rate than the control group and the Cy7-TCF group （ ⋅q=28.78，24.89，P< 0.05）. ConclusionCy7-TCF is effectivelytakenupbyMiaPaCa-2celsandislocalizedinlysosomes，andthrough itsphotothermal effect，itinduces the increase inthe levelof ROS incells，reduces cellviability，and promotes cellapoptosis.

[KEY WORDs]Pancreatic neoplasms； Photothermal therapy；Theranostic nanomedicine；Reactive oxygen species；Cel line，tumor；Nanoparticle drug delivery system

胰腺癌患者预后极差，5年生存率仅约 10%[1] （2只有约 20% 的患者在确诊时符合手术切除标准[2]，但术后复发率高达 80%13] 。（剩余12013字）