基于PI3K/Akt/NF-kB信号通路探讨黄连素对特应性皮炎大鼠皮肤病理变化的治疗作用

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The therapeutic efect of berberine on pathological changes of skin in rats with atopic dermatitis based on the Pl3K/Akt/NF- ΠκB signaling pathway

JIANG Su，LI Dongxia，LYU Xinxiang， CUI Yanhong，LYULiting

Departmentof Dermatologyand Venereology，Afliated HospitalofInerMongolia Medical University， Hohhot O050，Chi

Abstract：ObjectiveTo explore the therapeutic mechanismof berberine inatopic dermatitis （AD）rats basedon PI3K/ Akt/NF-kappa B signaling pathway.MethodsSixty adult male Wistarrats were randomlydivided into the blank group （normal rats）， the control group （AD model， 50mg/kg berberine treatment） and the experimental group （AD model， 200mg/kg berberinetreatment），with2Orats ineachgroup.Thelevelsof interleukin-4（IL-4），interleukin-13（IL-13）andtumor necrosis factor- α （TNF- α ）were determined by enzyme-linked immunosorbent assay （ELISA） at1d，7 dand14d of intervention. The protein levels of PI3K，p-PI3K，Akt，p-Akt and NF- κI 3p65 were detected by Western blot assay. Pathologicalchangesofratskintissue wereanalyzedbyHEstaining.ResultsAfterinterventionfor1d，7dand14d， serum levels of IL-4，IL-13，TNF- ∝ and PI3K，p-PI3K，Akt，p-Akt and NF-kappa B p65 were higherin the control group than those intheblank group（ P<0.05 ）.Afterinterventionfor7dand14d，thelevelsof theabove indicatorswerelower in the experimental group than those in the control group （ P<0.05 ）.After14 days of intervention，compared with the blank group，theskintissue ofrats inthecontrol groupand theexperimental groupshowedobvious pathologicalchanges，including thickening of epidermis layer，excesive keratinization of the stratum corneum，thickening of spinous layer anda large infiltrationof inflammatorycelsindermis.Thepathologicaldamageofratskin tissue was significantlyallviated inthe experimental group.ConclusionBerberinecan inhibittheactivationof PI3K/Akt/NF-kappa Bsignaling pathway，reduce serum level of inflammatory factors and reduce pathological damage of skin tissue in AD rats.

Keywords：dermatitis，atopic;berberine;phosphatidylinositol3-kinases;protooncogeneproteinsc-akt;NF-kappaB; cytokines; skin tissue

特应性皮炎（atopicdermatitis，AD）是一种以皮肤干燥、慢性瘙痒和复发性炎症为特征的慢性炎症性皮肤病1。（剩余12311字）