陆地棉gag基因家族的鉴定与分析

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Abstract：[Objective]Transposableelementsareamajordriverof genomeevolution，yetthefunctionsofthegaggenefamilyin uplandcotonremainunclear.Thisstudyaimedtoidentifymembersofthegaggenefamilyinuplandcotonandanalyze their structuralcharacteristics，evolutionaryelationshipsandexpresionpates toexploretheirpotentialolesinottongrowth， development，andstressresponses.[Methods]BasedontheTM-1reference genomeofuplandcotton，gaggenefamilyembers were identified using HMMER software，NCBI-CDDdatabaseandthePfamdatabase.Bioinformaticstoolswereemployed to analyze theirphysicochemical properties，subcellularlocalization，andchromosomaldistribution.Phylogenetictrees were constructed using ClustalXand MEGA X.Cis-acting elements in promoter regions were predicted using PlantCARE website， andLTRtransposon distribution wasanalyzed with RepeatMaskerandLTR_retriever.Transcriptomedata and quantiative real-time polymerase chainreaction were performedtoanalysis theexpressionpaterns and functionsof gag genes.[Results]A total of 166gag genes was identified in uplandcoton with significantly more genes inthe A sub-genome than in the D sub-genome.Phylogeneticanalysisdividedthefamilyintotwomajorsubfamilies（ninesubgroups）withdiversegenestructures， mostofwhich are located inLTR transposons.Promoter analysis revealed abundant cis-acting elements relatedtocoton growth，development，hormoneresponses，and stressadaptation.Expresionprofilingshowedthatcertaingenes，suchas

Ghir_A09Go13490and Ghir_D04G004460，werehighly expressed inspecifictisses（e.g.，ovules，fibers）orunderstre conditions（e.g，hightemperature，saltstress）.Conclusion]hegaggenefamilyexhibitssignificantdiversityinuplandcotton with its evolutionclosely linkedtoLTRtransposonactivity.Somemembersmayplayroles inabioticstressresponsesandseed development， offering potential targets for cotton genetic improvement.

Keywords： Gossypium hirsutum L.; gag gene; bioinformatics; expression pattern

转座子活动是生物基因组进化的重要驱动力，转座子不均等扩增调控同源基因转录、剪接和翻译，进而影响棉种谱系的特异分化。（剩余12934字）