GC-MS/MS法测定茶鲜叶中20种邻苯二甲酸酯类化合物

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Determination of 2O Phthalate Acid Esters in Tea Leaves by GC-MS/MS

SUN Yu1²， ZHOU Li2 ， ZHANG Xinfu1*， SUN Hezhi2*

1.ColegeoflantProtection，QingdaoAgriculturalUniversityQingdao266o9，Chia;2.ResearchCenterofQualityfetyfor AgriculturalProducts，TeaResearch Institute，ChineseAcademyofAgriculturalSciences，Hangzhou3loo8，China

Abstract： In this paper，an analytical method for the simultaneous determination of 20 phthalate esters in tea fresh leaves was developed by gas chromatography-tandem mass spectrometry （GC-MS/MS）.The phthalate esters in tea fresh leaves were extracted by 0.1% acetonitrile acetate， salted out with sodium chloride，cleaned up with a florisil solid phase extraction column， resolved with acetonitrile， separated by temperature programmed chromatography， monitored with multiple reaction monitoring （MRM） mode，and quantified by the external standard method. Through theoptimizationofsample extraction，purificationand chromatographicconditions，god linearityofthe targetcompounds was obtained in the concentration range of 0.010-2.0mg⋅kg-1 with the corresponding coefficient of determination R2>0.9955 .The average recoveries ranged from 70% to 118% at the three spiked levels of 0.010mg⋅kg-1 0.10mg⋅kg-1 and 1.0mg⋅kg-1 ，with the relative standard deviations （RSDs）of <17.8% .The limits of quantification were 0.010～0.050mg⋅kg-1 . The method is stable and reliable，and it can meet the needs of the determination of phthalate acid esters in tea fresh leaves.

Keywords： tea fresh leaves，gas chromatography-tandem mass spectrometry，phthalate acid esters

茶叶丰富的次生代谢物儿茶素、咖啡碱、茶氨酸和挥发物等赋予其独特的风味和健康益处[1]。（剩余14168字）