茶树生物钟CsLUX基因的鉴定及其对光合特性的影响

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Identification of Circadian Clock CsLUX Gene and Its Effects on Photosynthetic Characteristics in Tea Plants

SUN Mengzhen'， HU Zhihang1，YANG Kaixin1， ZHANG Jiaqi1， ZHANG Nan² XIONG Aisheng²， LIU Hui²， ZHUANG Jingl

1.MinistryofAgricultureandRuralAfairKeyLaboratoryofBologyandGermplasmEnhancementofHorticulturalCropsinEastChina TeaReseachioofiuregualeitygna;2ateKyb CropGenetics&Germplasm EnhancementandUtilization，NanjingAgriculturalUniversity，Nanjing21oo95，China

Abstract：Tea is a cash crop with large cultivated area in China，and its growth and development mechanism are strictly regulated by genes related to biological clock. In this study， the CsLUX gene was cloned using cDNA from tea cultivar‘Longjing 43， as template and sequence alignment and phylogenetic analysis were performed. Within two photoperiodic periods （ （48h） ，the relative expression of CsLUX gene was detected by RT-qPCR， and photosynthetic parameters，stomatal opening and chlorophyll contents in tea leaves were also determined.The total length of CsLUX gene is 951bp ， encoding a hydrophilic protein composed of 316 amino acids. The secondary structure is mainly composed of a -helix and random curling. CsLUX is closely related to CCA1，ELF3 and other key components of the biological clock. The variation trend of photosynthetic parameters in tea leaves at diferent time points was basically consistent with stomatal opening，and the value of chlorophyll SPAD was basically stable.The relative expression of CsLUX reached its peak under dark conditions and maintained a low expression level during the day in response to changes in circadian rhythm.The expression of CsLUX gene Was correlated with the rhythmic changes of photosynthetic parameters in two photoperiodic periods （48 h） in tea plants.

Keywords： tea plant， circadian clock， CsLUX， photosynthetic characteristics，expression analysis

茶树[Camellia sinensis（L.）O.Kuntze]作为一种栽培范围广泛的叶用经济作物，对光照条件具有独特而显著的适应性特征[1]。（剩余9702字）