基于CRISPR/Cas技术的肉类掺假检测研究进展

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Advances in Meat Adulteration Detection Based on CRISPR/Cas Technology

GUO Runze'， ZHANG Ying²， YANG Ruiqin1，*，SUN Qifan* （1. ， China， ， 2. ， ， ， China）

Abstract：Inrecent years，meatadulteration has become aprominentissuethreatening foodsafetyandtouchingonreligion， economy，and more，making itapresing social problem.While various detection methods exist for meatadulteration， traditional techniquesfall short inmetingthedemandforrapidandaurateon-sitedetection.Thus，developingrapidand accurate methods for on-site detection meat adulteration is greatsignificance.The clustered regularly interspaced short palindromic repeats （CRISPR）/CRISPR-asociated proteins （Cas））system，composed CRISPRand Cas，has the advantages trans-cleavage activityand high specificityandhas become an ultrasensitive nucleicacid detection tool.It showssignificant potential inmeatadulterationdetection.This paperreviewstheprosandcons theexisting methods for meat adulteration dtection，and introduces thecomposition，clasification，and mechanismaction the CRIPR/Cas system，witha focus onthe application CRIsPR/Cas-based technology in meat adulterationdetection.Italsodiscusses the advantages，challenges and future directions this technology.

Keywords： clustered regularly interspaced short palindromic repeats （CRIsPR）/CRIsPR-associated proteins;nucleic acid detection;meat adulteration

DOI：10.7506/rlyj1001-8123-20250714-229

中图分类号：TS207.3 文献标志码：A 文章编号：1001-8123（2026）07-0073-08 引文格式：

郭润泽，张颖，杨瑞琴，等.基于CRISPR/Cas技术的肉类掺假检测研究进展[J]：肉类研究，2026，40（7）：73-80. DOI：10.7506/rlyj1001-8123-20250714-229. http：/www.rlyj.net.cn

GUO Runze， ZHANG Ying，YANG Ruiqin，et al. Advances in meat adulteration detection based on CRISPR/Cas technology[J]. Meat Research，2026，40（7）：73-80.（in Chinese with English abstract） DOI：10.7506/rlyj1001-8123- 20250714-229.http：//www.rlyj.net.cn

肉类及其制品作为全球广泛消费的食品，富含蛋白质、脂肪、维生素及多种人体必需营养成分。（剩余21947字）