日粮添加百里香酚对羔羊肌纤维抗氧化能力的影响

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（1. 呼和浩特；2.内蒙古农业大学杨续金创新工作室，内蒙古呼和浩特010018；3.内蒙古农业大学动物科学学院，内蒙古呼和浩特 ）

Effect of Dietary Thymol Supplementation on the Antioxidant Capacity of Muscle Fibers in Lambs

ZHANG Yifei1，YUE Ling1，YANG Xujin12， WANG Hairong³，GAO Aiwul2* （1.CollegeofFoodScienceandEngineering，InnerongoliaAgricultural UniversityHohhotO10030，China; 2.Yang Xujin Innovation Studio，Inner Mongolia Agricultural University，HohhotO1o018， China; 3.College of Animal Science，Inner Mongolia Agricultural University，HohhotO1oo30，China）

Abstract： To explore the regulatory efect of oxidative stress on meat quality post-slaughter， six hybrid male lambs of Mongolian sheep and Duper sheep were selected and randomly divided into two groups： one as HC （fedon a basal dietcomposed of roughage mixed with forage and concentrated supplement）and the other as TG （fed on a basal diet supplemented with 0.3‰ thymol （based on the mass of the diet）.At the end of the feeding trial，the animals were slaughteredandlongissimus dorsisampleswerecolectedforanalysis ofantioxidant indicesandRNA sequencing （RNA-Seq）. It was foundthatdietarythymolwas effectivelydepositedinlamb meat，inhibiting theoxidationof myofibrillrproteinand fat， reducing reactive oxygen species levels，mitigating Ca2+ overload and mitochondrial lipid peroxidation， inhibiting the opening of mitochondrial permeabilitytransition pores，enhancing thereduction levelof cytochromec，andreducing the activitiesofcaspase-3andcaspase-9，thekeyenzymes inthe mitochondrialapoptoticpathway.ThroughRNA-Seq analysis， sevencandidate genesregulatingtheoxidativestressleveloflambmusclefiberswereselected，namelyFI6，PDL4，DGFB， IL6，MCL1，HMOX1，andFOXO1.Overall，themechanismbywhichdietarythymolenhances theantioxidantcapacityof lamb muscle fibers begins with the regulation of gene expression.

Keywords： thymol; lamb muscle fibers; oxidative stress; mitochondria;transcriptome; oxidation stability DOI：10.7506/rlyj1001-8123-20250718-234 中图分类号：TS251.1 文献标志码：A 文章编号：1001-8123（2026）07-0018-12

引文格式：

张义飞，岳灵，杨续金，等．日粮添加百里香酚对羔羊肌纤维抗氧化能力的影响[J].肉类研究，2026，40（7）：18-29. DOI：10.7506/rlyj1001-8123-20250718-234. htp：//www.rlyj.net.cn

ZHANG Yifei，YUELing，YANG Xujin，etal.Efectof dietary thymolsupplementationontheantioxidantcapacityof muscle fibers inlambs[J].MeatResearch，2026，40（7）：18-29.（inChinesewithEnglishabstract）DOI：10.7506/rlyj1001- 8123-20250718-234.http：//www.rlyj.net.cn

在畜牧生产实践中，羔羊饲养方式对其生长发育和健康状况具有重要影响，这种影响会通过其机体生理功能间接作用于肉品屠宰品质。（剩余27903字）