高效液相色谱法同时测定桂郁金中7种成分的含量

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中图分类号：R284.1 文献标志码：A 文章编号：1003-9783（2026）03-0531-06

doi：10.19378/j.issn.1003-9783.2026.03.015

Simultaneous Determination of Seven Components in Curcumae Radix by High Performance Liquid Chromatography

LI Zhaohui，LI Liangbo，QIN Xuelian，LI Chen，LIN Qinghua，ZENG Chunhui（Collge of Pharmacy，GuangxiUniversity of Chinese Medicine，Nanning 53O2Oo Guangxi，China）

Abstract：ObjectiveTo establish a method forthe simultaneous determinationof sevencomponents（aerugidol， demethoxycurcumin，curcumin，curcumenol， isocurcumenol，germacrone，and furanodiene） in Curcumae Radix based on high performance liquid chromatography （HPLC）.Methods HPLC analysis was performed using a Waters Symmetry C18 column （ 4.6mm×250mm ， 5μm ）.Agradient elution system consisting of acetonitrile（A）and- 0.2% （204号 phosphoric acid aqueous solution（B） was used as the mobile phase.The flow rate was O.8， ，the detection wavelength was set at 204nm ，the column temperature was maintained at ，and the injection volume was 10μL .Results The seven components in Curcumae Radix were well separated. The linear ranges for aerugidol， demethoxycurcumin，curcumin，curcumenol， isocurcumenol，germacrone，and furanodiene were 4.84-48.36，0.08- 3.36，0.16-3.25，3.20-32.00，0.32-3.22， 1.61-32.24，and 0.32-6.48μg⋅mL-1 ，respectively.All components exhibited excellent linear relationships （ r⩾0.9995 ）. The average recovery rates ranged from 96.37% to 108.47% ，with 1 RSD⩽2.14% . In the analysis of 22 batches of Curcumae Radix samples，the average contents of the aforementioned seven components were 308.39，14.88，25.30，328.72，21.83，152.00， and46.20μg∙g-1 ，respectively. Conclusion This method isaccurateandreliable，suitable for thequalityevaluationof Curcumae Radixand the quantitative determinationof itssevencharacteristiccomponents.Itcan provideascientific basis forthequalitycontrolof Curcumae Radix.

Keywords： Curcumae Radix；HPLC；content determination；quality evaluation

桂郁金为广西莪术Curcuma kwangsiensis S.G.LeeetC.F.Liang的干燥块根，是中药郁金的常见基源之一。（剩余6293字）