拟南芥FRO3基因突变体的构建及分析

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关键词拟南芥；FRO3；铁还原氧化酶；CRISPR/Cas9；基因编辑

中图分类号Q943.2 文献标识码A

文章编号 0517-6611（2025）21-0082-09

doi：10.3969/j. issn. 0517-6611. 2025.21. 016

AbstractObective]ToinvestigatethebologicalfunctionofFRO3.Metod]Alos-of-functionmutantusinggeneditingtechologywas constructedanditsoleintheregulationofironhomeostasiswasexamined.ResultProteinseqenceanalysisrevealedthatFOcotains conservedtructuraldomasharacteisicofoducgoidasstoccalngdmostratedtatFOexpresioisidcedy irondeficiencyiothosndlavsSublarcalizaalsdicatedtatteOproteinislocalditoode independentfro3mutantsweregeneratedbyCRIRPR/Cas9editing，achexhibitingprematureterminationofFRO3proteintranslationUnder irondeficiencytfro3utantsddotehbitaysigificantdierecsompadtoild-teplantsGeneexpresioaalysissodat thefro3mutantsddotsigficantlylterteexpressoofgeslatedtoonutakeandtrasport.CocusioO3mylig nificant role in iron homeostasis in Arabidopsis thaliana.

Key Words Arabidopsis thaliana ;FRO3;Ferric reductase oxidase; CRISPR/Cas9;Gene editing

铁是植物生长发育所必需的微量元素。（剩余20158字）