丝素蛋白水溶液制备工艺对其冻融再生多孔支架材料结构与性能的影响

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中图分类号：R318.08文献标志码：A

Effect of Silk Fibroin Aqueous Solution on Structure and Properties of Regenerated Porous Scaffold Materials Using Freeze-Thaw Method

WANG Yaxin， SHI Tianqi， WANG Qian， NING Wan’e， HUANG Jiwei

（1. College ofBiological and Chemical Engineering，2.Rainbow Modern Textile Industry College，3. Guangxi Zhuang Autonomous Region Engineering Research Center of Silk， Guangxi University of Science and Technology， Liuzhou ， Guangxi， China）

Abstract：Three typical solventsystems，calciumchloride-ethanol-water，lithiumbromide-water，andcalciumchlorideformicacid，were employed to disolve degummedsilk fibers.Theresulting silkfibroinsolutions weresubsequently subjectedto dialysis，filtration，andconcentration processes toobtain three distictsilk fibroin aqueoussolutions.Porous scafoldswerethen fabricated from each solutionusing thefreze-thaw method.The morphology，porosity，structure，thermal stability，and mechanical properties oftheresulting scaffolds were characterizedusing scanning electron microscopy （SEM）， X-raydifraction（XRD），Fourer-transfoinfraredspctrocopy（F-R），thermogravimetricaalysis（TGA），itial scaning calorimetry （DSC），and mechanical testing.Theresults indicated that the preparation methodsofthethree silk fibroinaqueous solutions exertedonlya minor influenceon the structure and propertiesof the freeze-thaw-derived porous scaffolds.AllscaffoldsexhibitedinterconnectedporousarchitecturesandastableSilkIconformation.However，thescafold derived from the calcium chloride-formic acid-based silk fibroin solution showed a higher β -sheet content and superior compressive mechanical properties.

Key words： calcium chloride-formic acid solvent system;silk fibroin aqueous solution;freeze-thaw method;porous scaffold material;Silk I structure

丝素蛋白（SilkFibroin，SF）是一种源于蚕丝的天然高分子聚合物，可大规模生产，且已获得美国药品监督管理局（FDA）批准认证[I。（剩余15330字）