盐胁迫下TaHsfA2-13对小麦 K⁺/Na⁺ 平衡的调控作用

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Abstract：To investigate the role of heat shock transcription factor gene TaHsfA2-13 in wheat salt tolerance， we analyzed the salt-stress responses of wild-type Cang 6005 and the TaHsfA2-13 knockout mutant tahsfa2-13. Seedling phenotype， K+/Na+ content and K+/Na+ ratio in roots and leaves were examined under salt stress. No difference on biomass accumulation between Cang 6005 and mutant tahsfa2-13 was observed under normal growth conditions. Under 200mmol/LNaCl treatment conditions，tahsfa2-13 exhibited reduced plant height， increased leaf withering，and significantly lower survival rates after stress recovery.In tahsfa2-13 VS Cang 6005，higher Na+ accumulation in leaveswas found，and lower K+ and Na+ content in roots were detected， leading to significantly lower K+/Na+ ratios in both roots and leaves of the mutant. Through transcriptome sequencing analysis，9369 and 2433 differentially expressed genes （DEGs）were identified in the leaves and roots，respectively. GO analysis highlighted enrichments of DEGs in transcription，cytoplasmic，metal-ion binding and transmembrane ion transporters.Further analysis of the transmembrane ion transporters revealed that several genes of the family TaHAK and TaHKT family，which are high-affinity transporter proteins involved in K+ ion transport. Five TaHAK genes and five TaHKT genes were up-regulated，and three TaHAK genes and one TaHKT gene were down-regulated in leaves under salt stress Four TaHKT genes and two TaHKT genes were down-regulated in roots under salt stressThese results suggest that TaHsfA2-13 modulates salt tolerance by regulating the transcription of potassium transporter-related genes and maintaining ion homeostasis under stress.This study provides a theoretical basis for elucidating the molecular mechanism of TaHsfA2-13insalt stress.

Keywords：wheat; TaHsfA2-13;mutant; salt stress;transcriptome sequencing; K+/Na+ homeostasis

我国是世界上土壤盐渍化较严重的国家，据不完全统计，我国约有1亿 hm2 的盐碱地，占全球盐碱地总面积的 10% 以上[1]。（剩余23369字）