新型茶叶/茶枝柑融合纳米囊泡的构建及其抗炎抗肿瘤活性评价

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中图分类号：S571.1；TS272.4 文献标志码：A 文章编号：1000-369X（2026）02-0343-16

Abstract： Plant exosome-like nanovesicles （ELNVs），as inherent components of plants，are nanoscale vesicles secreted by plant cells. They are inherently rich in bioactive compounds，and their vesicular structure makes them ideal"green”drug delivery vehicles.To overcome the limitations of single-source plant ELNVs，this study employed membrane fusion technology to combine tea ELNVs and Citrus reticulata ‘Chachiensis’ELNVs for the first time， creating novel fusogenic nanovesicles.We subsequently characterized the physicochemical properties of novel fusogenic nanovesicles，elucidated their fundamentalcomposition，assessed their stabilities in simulated digestive fluids，and evaluated their antioxidant，anti-inflammatoryand anti-tumoractivities through celular assays.The results idicate that the novel fusogenic nanovesicles are a reconstituted liposome integrating multiple functional components，including teapolyphenols （catechins），hesperetin，hesperidin，nobiletin，queretin，active phospholipids and proteins. They exhibit a spherical morphology witha phospholipid bilayer structure， demonstrating good dispersibility with uniform particle size.Additionall，the novel fusogenic nanovesicles demonstrate excellent stabilityin both simulated gastric and intestinal fluids. Cell-based experimental results show thatthe novel fusogenic nanovesicles significantly aleviated lipopolysaccharide （LPS）-induced oxidative stress damage and inflammatory responses in RAW264.7 cels， while exhibiting superior antitumor eficacy. The findings of this study not only provided novel insights into theapplication of natural plant ELNVs，butalso established a scientific foundation for developing innovative multifunctional oral nutraceuticals.

Keywords： plant exosome-likenanovesicles （plantELVs），reconstituted liposomes，anti-inlammatoryntioidant， antitumor

炎症性肠病（Inflammatoryboweldisease，IBD）是一种累及回肠、直肠、结肠的特发性肠道炎症性疾病，主要包括溃疡性结肠炎（Ulcerativecolitis，UC）和克罗恩病（Crohn'sdisease，CD）两种类型[1-3]。（剩余21373字）