望海茶1号’配套扦插繁育技术和不定根转录特性分析

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中图分类号：S571.1；S339.4 文献标志码：A文章编号：1000-369X（2026）02-0207-14

Abstract：‘Wanghaicha 1' is a newly bred green tea cultivar origining from the local population in Ninghai county. To establish an eficient breeding system，a comparative cutting experiment of long and short branch cutings in lightweight non-woven seedling bags，and a short branch field mulching cuting experiment were set upat the Shengzhou base.Theresults show that therootingrate of short branch cutings ofthiscultivar Was higher than that of the control‘Longjing 43’， with beter growth and greater tolerance to high temperatures and drought. Moreover， the long branch cuttings can reach the nurserystandard after 77 days.The method of short branch cuttings covered with black non-woven fabric film on the sedbed can efectively reduce the frequency of manual weeding. And through Ilumina HiSeq platform，transcriptome sequencing of the root system of‘Wanghaichal’was performed.

Compared with the control ‘Longjing 43∘ ，107 differentially expressed genes related to plant hormones were screened. It is speculated that the synergistic regulation of these genes may be the molecular basis for the developed root system of this culitvar. This study preliminarily obtained107 differentially expressed genes related to plant hormones in the adventitious roots of‘Wanghaichal'. The established cuting mode of ‘early emergence of long branch from the nursery application of black plastic film to improve qualityand eficiency'can shorten the seedling period by 15.4% and reduce weed control costs by 85.7% ， providing theoretical basis and technical support for the industrial promotion of new cultivar.

Keywords： new tea cultivar， breeding， cutting techniques， adventitious root， transcriptome

茶树（Camelliasinensis）是我国重要的经济作物之一，其品质和产量将直接影响茶产业的市场竞争力[1]。（剩余14545字）