基于孟德尔随机化方法探讨473种肠道菌群与百日咳因果关系

打开文本图片集

中图分类号：R516.6 文献标志码：A DOI ： 10.3969/j .issn.1003-1383.2025.08.008

Investigation on causal relationship between 473 gut microbiota and pertussis based on Mendelian randomization method

JIANGXijuan'，LIAO Bicai² （1.Department of Pediatrics，Beihai Tuberculosis Preventionand Treatment Hospital， Beihai 536o，Guangxi，China; 2.DepartmentofPediatrics，Guangxi Zhuang AutonomousRegionEthnic Hospital，Nanning53oo，Guangxi，China）

【Abstract】ObjectiveToinvestigate causal relationship between 473gut microbiotaandpertusis by genome-wide Mendelianrandomization method.MethodsGut microbiotadata were derived from genome-wideassociation study（GWAS）， whichincludedatotalof5959samplescovering 473gutmicrobiotaspecies.Pertussisdata wereextractedfromtheFinnGenR9 database（147casesand332343controls）.Inverse variance weighted（IVW）methodwasusedforbidirectionaltwo sample Mendelian randomization analysis to evaluate the causal effect of gut microbiota on pertusis，and Cochran's Q test，MR-Egger intercepttest，andMR-PRESSO wereusedtoexamineheterogeneityandpleiotropy.ResultsAtotalof 2Ogutmicrobiota were identifiedascausalllinked topertusis.Among them，11microbiota exhibitedprotectiveefects，withUBA7177showingthe strongest protection （OR=0.1404，95% CI ： 0.0240 - 0.8214， P=0.0294 ）；9 species increased the risk of pertussis，with Lawsonella spOO2161175 presenting the highest risk（ OR=15.2697 ，95%CI： 2.3443-99.4593，P=0.0044）. Conclusion

Acausal relationship exists between gutmicrobiotaandpertusis，and targeted modulationof specificmicrobiotamayffer novel strategies for the prevention and treatment of pertussis.

【Keywords】 gut microbiota；pertussis；Mendelian randomization；causal relationship；genome-wide association study （GWAS）

百日咳是一种由百日咳杆菌（bordetellapertus-sis）引起的急性呼吸道传染病，其特征性症状包括剧烈的咳嗽发作和特有的“鸡鸣”样吸气声，在儿童群体中尤为显著[1]。（剩余9294字）