基于离子液体与微波辅助的川续断提取液抑菌作用及机制研究

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中图分类号：TS210.9 文献标志码：A 文章编号：1671-8755（2025）02-0025-08

Study on the Antibacterial Effect and Mechanism of Dipsacus Asper Extract Based on Ionic Liquid and Microwave-assisted Method

ZOU Jing1 ，KANG Jianqing'， ZHOU Mengjiao²， ZHANG Yu' ，KANG Ming'，LIANG Xiaofeng1，²

（1. School of Materials and Chemistry，Southwest University of Science and Technology，Mianyang 621010， Sichuan， China; 2. Mianyang Key Laboratory of Development and Utilization of Traditional Chinese Medicine Resources， Sichuan College of Traditional Chinese Medicine，Mianyang 621olo， Sichuan，China）

Abstract：Using Dipsacus as the raw material，the extract was obtained through ionic liquid and microwave-assisted extraction technology.Antibacterial experiments and active component analysis of the extract were conducted，and the antibacterial mechanism was discussed.The results show that the Dipsacus extract exhibits antibacterial effects against Escherichia coli（ E . coli），Staphylococcus aureus （S. aureus），and Salmonella paratyphi β （ S .paratyphi β ），with its inhibitory activity being concentration-dependent. The inhibition zone diameters for E . coli，S.aureus，and S . paratyphi β are 27 mm，39 mm，and 19mm ，respectively. The minimum inhibitory concentration （MIC）and minimum bactericidal concentration（MBC） against S. aureus are determined to be 3.1250mg/mL and 6.250 0mg/mL ， respectively. For E ： coli，the MIC and MBC values are 3.1250mg/mL and 6. 250 0mg/mL ， respectively. For S ：paratyphi β ，MICis 6.250 0mg/mL and MBC is 12.500 0mg/mL . Chromatographic analysis reveals the presence of antibacterial components in Dipsacus extract，including chlorogenic acid，caffeic acid，and asperosaponin VI. Morphological observations suggest that the antibacterial mechanism may involve disruption of bacterial cell wals and membranes.These findings provide valuable references for the development of antimicrobial agents from Dipsacus.

Keywords： Dipsacus asper extract；Antibacterial constituent； Antibacterial activity； Antibacterialmechanism

植物中的皂苷类、酚酸类成分（绿原酸、咖啡酸等）对多种革兰阳性和革兰阴性细菌均具有显著的抑菌作用[1-4]，其抑菌机制可能与降解细菌细胞壁结构、破坏细胞膜完整性导致胞内物质外泄并引发细菌死亡相关[5]

川续断（DipsacusasperWall.exHenry）是川续断科多年生草本植物的根，富含皂苷成分，以“续折接骨"之功效而闻名[6，是一种重要的中药材。（剩余10163字）