板栗2-ODD基因家族的鉴定及其在干旱胁迫下的表达分析

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Identification of Chinese Chestnut -ODD Gene Family and Their Expression Analysis under Drought Stress

Zeng Yangjuan 1，2，3 ，Yang Yong23，GuoJianghu2，ZhangHai'e2，CaFe （1.Collegeof HorticulturalScienceandTechnology，Hebei Nrmal UniversityofScienceandTechnology，inhuangdao066004， China;2.Enginering Research Centerof Chestnut Industry Technology，Ministryof Education，Qinhuangdao O66004，China; 3.KeyLaboratoryof CharacteristicHorticultural Germplasm Miningand Innovative Utilizationof HebeiProvince，Qinhuangdao 066004，China;4. Hebei Province Chestnut Industry Collaborative Innovation Center， Qinhuangdao O66004，China）

AbstractThe 2-ODD gene family is the second largest enzyme family in the plant and has an important role in regulating plant response to stresses.Using bioinformatics methods，we identified 123 Cm2-ODD genes from the chestnut genome，their chromosomal location，protein physicochemical properties，conserved motifs， gene structure，collinear genes and cis-acting elements，and the expresson under drought stress were analyzed. The results found that all the Cm2 -ODD family proteins contained the conserved 2OG-Fe II _Oxy and DIOX_N domains，and were hydrophilic proteins. Most members of Cm2-ODD gene family had conserved motifs of Motif 1 to Motif 9，and only a part members had Motif 10，indicating that the function of Cm2-ODD proteins might be relatively conserved.Collinearity analysis found that the Cm2-ODD family genes within the chestnut genome might be under purifying selection，and a masive replication of the 2-ODD gene family might occur after the diferentiation of monocotyledonous and dicotyledonous plants.Codon preference analysis showed that Cm2-ODD family genes preferred to end with A or U.The result of cis-acting element analysis showed that Cm2-ODD family genes contained more abscisic acid and drought response elements，speculating that its function might be asociated with drought stress.Meanwhile，transcriptome analysis proved that the expresion levels of 13 members of the Cm2-ODD gene family were significantly affcted by drought stress，among which， the expresson of Cm2-ODD19 and Cm2-ODD16 genes was up-regulated.

KeywordsChinese chestnut； Cm2-ODD gene family； Bioinformatics analysis；Drought stress； Geneexpression

板栗（Castaneamollissima）属于壳斗科（Fa-gaceae）栗属（Castanea）多年生木本植物，是我国重要的木本粮食[1-2]，果实富含大量营养成分[3]，一直深受国内消费者的喜爱[4]。（剩余11924字）