二肽基肽酶-4在瘢痕疙瘩中的表达情况研究

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[摘要]目的：探讨二肽基肽酶-4（DPP4）在瘢痕疙瘩中的表达情况。方法：分别获取切除术后的瘢痕疙瘩组织及正常皮肤组织，然后通过蛋白免疫印迹方法、定量聚合酶链反应，比较二者DPP4基因在蛋白和mRNA水平上表达的差异。结果：与正常皮肤组织相比，DPP4蛋白在瘢痕疙瘩组织中的表达高于正常皮肤，差异有统计学意义（P

[关键词]瘢痕疙瘩;纤维化;二肽基肽酶-4;实时聚合酶链反应;蛋白免疫印迹;基因表达;成纤维细胞

[中图分类号]R619+.6    [文献标志码]A    [文章编号]1008-6455（2022）01-0087-03

Study on the Expression of Dipeptidyl Peptidase-4 in Keloids

LIANG Zhongyao1，CEN Ying2

（1.West China Clinical Medical College of Sichuan University，Sichuan University，Chengdu 610000，Sichuan，China;2.Department of Plastic and Aesthetic Surgery，West China Hospital，Sichuan University，Chengdu 610000，Sichuan，China）

Abstract： Objective To explore the expression of dipeptidyl peptidase-4 in keloids. Methods The keloid tissue and normal skin tissue after resection were obtained， and then the differences in the expression of DPP4 gene between the two were compared by Western blotting and quantitative polymerase chain reaction. Results Compared with normal skin tissue， the expression of DPP4 protein in keloid tissue was higher than that in normal skin， and the difference was statistically significant （P

Key words： keloid; fibrosis; Dipeptidyl Peptidase-4; real-time polymerase chain reaction; blotting western; gene expression; fibroblast

瘢痕疙瘩（keloid）是整形外科中一类常见的皮肤纤维化疾病，发病机制大多与胶原纤维过度修复增生有关，其主要特征表现为高出于皮肤表面、侵袭性生长和容易复发。（剩余4722字）