秋水仙素诱导阳光玫瑰葡萄多倍体的效果研究

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中图分类号：S663.1 文献标志码：A 文章编号：1002-2910（2025）02-0038-05

Study on the effects of colchicine-induced polyploidization in Shine Muscat grapes

ZHANGShihao， SUNXinru，FENG Haonan，YAO Yuxin* （College of Horticulture Science and Engineering， Shandong Agricultural University， Tai'an，Shandong271018，China）

Abstract： The shoot segments of Shine Muscat grapes were used as materials， and 0 . 2 % 0 . 4 % ， 0 . 8 % ，and 1 . 2 % colchicine solutions were applied to treat the shoot with different times by the cotton immersion and soaking methods， the study was aimed to obtain the tetraploid grapes. The results showed that soaking grape branch segments in 0 . 4 % colchicine （ and 1 % dimethyl sulfoxide （ 2 0 0 m L ）for 6 days resulted in the greatest mutagenic effect， with a lethal rate of 5 0 . 0 0 % （20号 and a mutation rate of 6 . 6 0 % ， respectively. A total of 12 tetraploid and 2 mixed polyploid plants of the Sunshine Rose were obtained; The cotton soaking method with 1 . 2 % colchicine 2 0 0 m L + 1 % dimethyl sulfoxide 2 0 m L cotton soaked grape branch segments for 6 days showed the greatest mutagenic effect， with a lethal and mutation rate of 2 5 . 0 0 % and 6 . 0 0 % ， respectively.

Key words： grape; Shine Muscat; colchicine; induce; polyploidy

多倍体作物具有产量高、器官巨大、抗病抗逆能力强等特性，生产上有广泛推广应用[1。（剩余6262字）