升清降浊胶囊通过抑制NLRP3炎症小体激活改善糖尿病肾病的机制研究

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中图分类号：R285.5 文献标志码：A 文章编号：1003-9783（2025）03-0376-08

doi：10.19378/j.issn.1003-9783.2025.03.007

Mechanism of Shengqing Jiangzhuo Capsules in Improving Diabetic Nephropathy by Inhibiting the Activation of NLRP3 Inflammasome

YUYanna，DONG Xin，WANG Liangliang，TANG Shuifu（TheFirst Afiliated Hospital ofGuangzhou Universityof Chinese Medicine，Guangdong Clinical Research Institute of Traditional Chinese Medicine，Guangzhou Guangdong，China）

Abstract：Objective To explore the mechanism of Shengqing Jiangzhuo Capsules （SQJZC）in improving diabetic nephropathy（DN）.Methods DN model was established by intraperitoneal injectionof streptozotocin in C57/BL6 mice. Thechanges ofblood glucose andbody massof mice were detected every week.After8 weeks of treatment，the levels of urine total protein（UTP），serum creatinine（Scr）and blood urea nitrogen（BUN）were detected.Hematoxylineosin staining （HHE）and Periodic Acid-Schiff staining（PAS）were used to detect the changes of renal structure. The changes of inflammatory factors in kidney and serum were detected byqPCRand ELISA.Western Blot wasused to detect the changes of renal injury-related markers and NOD-like receptor pyrin domain-containing 3（NLRP3） inflammasome components.Results SQJZC had no significant effect on blood glucose and body massof mice.HE and PAS staining results showed that SQJZC treatment improved renal tubular vacuolar degeneration and necrosis，reduced brush border sheddingand fiberdeposition.Theresults offurther studyshowed that SQJZC treatmentcould significantly reduce the levels of UTP，Scr，BUN and serum inflammatory factors in diabetic nephropathy mice （ P<0.01 ）.Western

Blotresults showedthatSQJZC interventionreduced renalapoptosis-related factors and kidney injury molecule1（Kim-1） levels.The expressions of NLRP3，apoptosis-assciated speck-like protein containing a CARD（ASC）and cysteinyl aspartate specific proteinase -1 （Caspase ⋅-1 ）were inhibited（ P<0.05，P<0.01， . Conclusion SQJZC can improve renal injury in diabetic nephropathy，which may be achieved by inhibiting NLRP3 inflammasome.

Keywords： Shengqing Jiangzhuo Capsules； diabetic nephropathy； NLRP3 inflammasome；mice

糖尿病肾病（Diabeticnephropathy，DN）是糖尿病的一种严重并发症，是导致终末期肾病（End-stagerenaldisease，ESRD）的主要原因[。（剩余10598字）