蒲公英叶斑病菌鉴定及生物防治药剂筛选

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中图分类号：S435.6 文献标识码：A文章编号：2097-1354（2025）04-0056-10

Identification of Leaf Spot Disease Pathogens of Taraxacum mongolicum and Screen of Biological Control Agents

LIU Yapeng， MA Mei， QI HexingCollege of AgricultureandAnimal Husbandry，Qinghai University，Xining 810016，China

Abstract： Leaf spot disease is one of the common diseases of Taraxacum mongolicum，which can lead to a decrease in the yield and quality of T . mongolicum when it is severe. To further identify the species of pathogens causing dandelion leaf spot disease and screen out green and efficient biological prevention and control agents， the single spore isolation method was used to isolate the pathogen of dandelion leaf spot. A total 3 strains were obtained，which were determined as Stagonos poropsis chrysanthemi by morphological observation and molecular biology dentification. The mycelial growth inhibition method and confrontation culture method were used to further determine the sensitivity of nine biological agents to S . chrysanthemi and the Alternaria solani which could caused dandelion leaf spot disease obtained earlier. The results showed that，among the biosimilar extracts kind of fungicides，matrine had the best inhibitory effect on A . solani and S.chrysanthemi，with EC50 value of 0.05g/L and 0.042g/L ，respectively. Among the antagonistic bacteria fungicides，Bacillus polymyxa had the best inhibitory effect on A . solani and S. chrysanthemi，with inhibition zone widths of 0.80-1.43cm and 1.00 -1.87cm ，respectively. This study is the first report of S . chrysanthemi caused leaf spot disease in T . mongolicum，and successfully screened out the efficient biological control agents， which layed a foundation for the biological control of T . mongolicum leaf spot disease.

Key words： Taraxacum mongolicum; leaf spot disease; Stagonosporopsis chrysanthemi; biological control

蒲公英（Taraxacum mongolicum Hand.-Mazz.）别名黄花地丁、婆婆丁、华花郎等，是菊科多年生草本植物[1]，药食两用，已被列为中国中药保护植物，主要分布在中国西南及华北等地[2]。（剩余9679字）