阴阳蛋白1对膀胱癌细胞增殖和迁移的影响及机制

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Effects and mechanisms of Yin Yang 1 on the proliferation and migration of bladder cancer cells

JI Meng1 ，TU Sheng²，WANG Gang？，QIAN Kaiyu³，XIAO Yu3.4

（1.Department of Laboratory Medicine，2.Department of Urology，3.Department of Biological Repositories， Zhongnan Hospital of Wuhan University，Wuhan 430071；4.Human Genetic Resources Preservation Center of Hubei Province，Wuhan 430071，China）

ABSTRACT：ObjectiveTo explore the effects of Yin Yang 1（YYl）on the proliferationand migration of blader cancer cells and investigate the underlying mechanisms，soas toprovidereference forthe preventionandtreatmentof this disease. MethodsThe expression patterns of YY1 incommon genitourinary tumors and their asociations with the prognosis were analyzed using data from The Cancer Genome Atlas （TCGA）and the Gene Expression Omnibus （GEO）.The eficiency of YY1 knockdown and overexpresion in bladdercancercellines（T24and UM-UC-3）wasconfirmed withquantitativereverse transcription PCR（qRT-PCR）and Westernblotig.Cellproliferation andmigration wereasessedusing methylthiazolyldiphenyl-tetrazolium bromide（MTT）andTranswellasays.RNA sequencing followedbybioinformatics analyses，including GeneOntology（GO），Kyoto Encyclopediaof Genes and Genomes（KEGG），and Gene SetEnrichment Analysis （GSEA），was conducted to predict potential mechanisms.TheqRT-PCR and rescue experiments were performed to validate whether YY1 exerted its effects via theE2F1signaling pathway.ResultsYYl wassignificantlyoverexpresed in bladdercancer compared toother genitourinary tumors and was associated with higher tumorgradeand poorerprognosis（ P< 0.05）.Functional assys demonstratedthatYYlpromoted the proliferationandmigration of bladdercancer cels. Transcriptome analyses revealed that YYl might regulate these processs through the E2F signaling pathway.Moreover， overexpresionofE2Flpartiallyreversedthe inhibitory effectsofYY1knockdownon bladdercancercell proliferationand migration.ConclusionYYlis upregulated in bladdercancer and is closely assciated with tumor gradeandunfavorable prognosis.It may facilitate tumor cellproliferation and migration by modulating the E2F1 signaling pathway.

KEY WORDS： bladder cancer;Yin Yang 1；proliferation；migration

中图分类号：R737.14 文献标志码：A

DOI：10.3969/j.issn.1009-8291.2025.08.012

膀胱癌是最常见的泌尿系统恶性肿瘤之一，根据世界卫生组织预测，至2040年，膀胱癌的全球年新发病例预计将达到120万例，给社会带来巨大医疗负担[1。（剩余16285字）